KIF18A (NM_031217) Human Over-expression Lysate

Lysate of KIF18A over-expression cell lines, WB control

Transient overexpression lysate of kinesin family member 18A (KIF18A)

Product Info Summary

SKU: LS081930
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of kinesin family member 18A (KIF18A)

Product Name

KIF18A (NM_031217) Human Over-expression Lysate

See all KIF18A products

SKU/Catalog Number

LS081930

Size

100 µg

Description

Transient overexpression lysate of kinesin family member 18A (KIF18A)

Storage & Handling

Cite This Product

KIF18A (NM_031217) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS081930)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for KIF18A (Source: Uniprot.org, NCBI)

Uniprot ID

Q8NI77

Gene ID

81930

Gene Name

KIF18A

Full Name

Kinesin-like protein KIF18A

Weight

102.281kDa

Superfamily

TRAFAC class myosin-kinesin ATPase superfamily

Alternative Names

DKFZP434G2226; kinesin family member 18A; kinesin-like protein KIF18A; Marrow stromal KIF18A; MS-KIF18A KIF18A MS-KIF18A, PPP1R99 kinesin family member 18A kinesin-like protein KIF18A|protein phosphatase 1, regulatory subunit 99

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on KIF18A, check out the KIF18A Infographic

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