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Product Info Summary
| SKU: | LS121391 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of keratin 74 (KRT74) |
Product info
Product Name
KRT74 (NM_175053) Human Over-expression Lysate
SKU/Catalog Number
LS121391
Size
100 µg
Description
Transient overexpression lysate of keratin 74 (KRT74)
Storage & Handling
Cite This Product
KRT74 (NM_175053) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS121391)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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KRT74 Human Over-expression Lysates NM_175053.
Western blot validation of overexpression lysate (LS121391) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with KRT74 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for KRT74 (Source: Uniprot.org, NCBI)
Gene Name
KRT74
Full Name
Keratin, type II cytoskeletal 74
Weight
57.865kDa
Superfamily
intermediate filament family
Alternative Names
HTSS2; K6IRS4; KRT5C; KRT6IRS4; KRT74 keratin 74 KRT74 ADWH, HTSS2, HYPT3, K6IRS4, KRT5C, KRT6IRS4 keratin 74 keratin, type II cytoskeletal 74|CK-74|K5C|K74|cytokeratin-74|keratin 5c|keratin 6 irs4|keratin 74, type II|type II inner root sheath-specific keratin-K6irs4|type-II keratin Kb37
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on KRT74, check out the KRT74 Infographic
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In this infographic, you will see the following information for KRT74: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For KRT74 (NM_175053) Human Over-expression Lysate (LS121391)
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