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Product Info Summary
|Description:||Transient overexpression lysate of acidic (leucine-rich) nuclear phosphoprotein 32 family, member E (ANP32E), transcript variant 1|
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LANPL (ANP32E) (NM_030920) Human Over-expression Lysate
Transient overexpression lysate of acidic (leucine-rich) nuclear phosphoprotein 32 family, member E (ANP32E), transcript variant 1
Storage & Handling
Cite This Product
LANPL (ANP32E) (NM_030920) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS081611)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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LANPL (ANP32E) Human Over-expression Lysates NM_030920.
Western blot validation of overexpression lysate (LS081611) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ANP32E (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for ANP32E (Source: Uniprot.org, NCBI)
Acidic leucine-rich nuclear phosphoprotein 32 family member E
acidic (leucine-rich) nuclear phosphoprotein 32 family, member E; LANP-like protein; LANP-LLANPLacidic leucine-rich nuclear phosphoprotein 32 family member E; leucine-rich acidic nuclear protein like; MGC5350 ANP32E LANP-L, LANPL acidic nuclear phosphoprotein 32 family member E acidic leucine-rich nuclear phosphoprotein 32 family member E|LANP-like protein|acidic (leucine-rich) nuclear phosphoprotein 32 family, member E|leucine-rich acidic nuclear protein like*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on ANP32E, check out the ANP32E Infographic
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No publications found for LS081611
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