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Product Info Summary
|Description:||Transient overexpression lysate of late cornified envelope 3D (LCE3D)|
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LCE3D (NM_032563) Human Over-expression Lysate
Transient overexpression lysate of late cornified envelope 3D (LCE3D)
Storage & Handling
Cite This Product
LCE3D (NM_032563) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084648)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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LCE3D Human Over-expression Lysates NM_032563.
Western blot validation of overexpression lysate (LS084648) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with LCE3D (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for LCE3D (Source: Uniprot.org, NCBI)
Late cornified envelope protein 3D
Late cornified envelope protein 3D LCE3D LEP16, SPRL6A, SPRL6B late cornified envelope 3D late cornified envelope protein 3D|late envelope protein 16|small proline rich-like (epidermal differentiation complex) 6A|small proline rich-like (epidermal differentiation complex) 6B|small proline-rich-like epidermal differentiation complex protein 6A|small proline-rich-like epidermal differentiation complex protein 6B*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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