LEC (CCL16) (NM_004590) Human Over-expression Lysate

Lysate of CCL16/HCC-4/LEC over-expression cell lines, WB control

Transient overexpression lysate of chemokine (C-C motif) ligand 16 (CCL16)

Product Info Summary

SKU: LS006360
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of chemokine (C-C motif) ligand 16 (CCL16)

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Product Name

LEC (CCL16) (NM_004590) Human Over-expression Lysate

See all CCL16/HCC-4/LEC products

SKU/Catalog Number

LS006360

Size

100 µg

Description

Transient overexpression lysate of chemokine (C-C motif) ligand 16 (CCL16)

Storage & Handling

Cite This Product

LEC (CCL16) (NM_004590) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006360)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for CCL16 (Source: Uniprot.org, NCBI)

Gene Name

CCL16

Full Name

C-C motif chemokine 16

Weight

13.6kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

C-C motif chemokine 16; CCL16; chemokine (C-C motif) ligand 16; Chemokine CC-4; Chemokine LEC; CKb12; HCC4; HCC-4; HCC-4Liver-expressed chemokine; LCC-1Lymphocyte and monocyte chemoattractant; LEC; liver CC chemokine-1; LMC; LMCSmall-inducible cytokine A16; monotactin-1; Mtn-1; NCC-4; NCC4IL-10-inducible chemokine; NCC-4ILINCK; new CC chemokine 4; SCYA16MGC117051; SCYL4; small inducible cytokine subfamily A (Cys-Cys), member 16 CCL16 CKb12, HCC-4, ILINCK, LCC-1, LEC, LMC, Mtn-1, NCC-4, NCC4, SCYA16, SCYL4 C-C motif chemokine ligand 16 C-C motif chemokine 16|IL-10-inducible chemokine|chemokine (C-C motif) ligand 16|chemokine LEC|liver CC chemokine-1|liver-expressed chemokine|lymphocyte and monocyte chemoattractant|monotactin-1|new CC chemokine 4|small inducible cytokine subfamily A (Cys-Cys), member 16|small-inducible cytokine A16

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on CCL16, check out the CCL16 Infographic

CCL16 infographic

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In this infographic, you will see the following information for CCL16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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