MAGIX (NM_024859) Human Over-expression Lysate

Lysate of MAGIX over-expression cell lines, WB control

Transient overexpression lysate of MAGI family member, X-linked (MAGIX), transcript variant 1

Product Info Summary

SKU: LS079917
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of MAGI family member, X-linked (MAGIX), transcript variant 1

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Product Name

MAGIX (NM_024859) Human Over-expression Lysate

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SKU/Catalog Number

LS079917

Size

100 µg

Description

Transient overexpression lysate of MAGI family member, X-linked (MAGIX), transcript variant 1

Storage & Handling

Cite This Product

MAGIX (NM_024859) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS079917)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for MAGIX (Source: Uniprot.org, NCBI)

Uniprot ID

Q9H6Y5

Gene ID

79917

Gene Name

MAGIX

Full Name

PDZ domain-containing protein MAGIX

Weight

35.273kDa

Alternative Names

FLJ21687; MAGI family member, X-linked; X chromosome MAGIX JM10, PDZX MAGI family member, X-linked PDZ domain-containing protein MAGIX|PDZ domain containing, X chromosome

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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