MED25 (NM_030973) Human Over-expression Lysate

Lysate of MED25 over-expression cell lines, WB control

Transient overexpression lysate of mediator complex subunit 25 (MED25)

Product Info Summary

SKU: LS081857
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of mediator complex subunit 25 (MED25)

Product Name

MED25 (NM_030973) Human Over-expression Lysate

See all MED25 products

SKU/Catalog Number

LS081857

Size

100 µg

Description

Transient overexpression lysate of mediator complex subunit 25 (MED25)

Storage & Handling

Cite This Product

MED25 (NM_030973) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS081857)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for MED25 (Source: Uniprot.org, NCBI)

Uniprot ID

Q71SY5

Gene ID

81857

Gene Name

MED25

Full Name

Mediator of RNA polymerase II transcription subunit 25

Weight

78.171kDa

Superfamily

Mediator complex subunit 25 family

Alternative Names

ACID1; Activator Interaction Domain-Containing Protein 1; Activator-Recruited Cofactor 92 KDa Component; ARC/Mediator Transcriptional Coactivator Subunit; ARC92; BVSYS; CMT2B2; Mediator Complex Subunit 25; Mediator Of RNA Polymerase II Transcription Subunit 25; Mediator Of RNA Polymerase II Transcription, Subunit 25 Homolog (S. Cerevisiae); Mediator Of RNA Polymerase II Transcription, Subunit 25 Homolog; P78; TCBAP0758 MED25 ACID1, ARC92, BVSYS, CMT2B2, P78, PTOV2, TCBAP0758 mediator complex subunit 25 mediator of RNA polymerase II transcription subunit 25|ARC/mediator transcriptional coactivator subunit|activator interaction domain-containing protein 1|activator-recruited cofactor 92 kDa component|mediator of RNA polymerase II transcription, subunit 25 homolog|prostate-derived protein 78 kDa

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on MED25, check out the MED25 Infographic

MED25 infographic

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In this infographic, you will see the following information for MED25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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