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Product Info Summary
|Description:||Transient overexpression lysate of mediator complex subunit 25 (MED25)|
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MED25 (NM_030973) Human Over-expression Lysate
Transient overexpression lysate of mediator complex subunit 25 (MED25)
Storage & Handling
Cite This Product
MED25 (NM_030973) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS081857)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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MED25 Human Over-expression Lysates NM_030973.
Western blot validation of overexpression lysate (LS081857) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MED25 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for MED25 (Source: Uniprot.org, NCBI)
Mediator of RNA polymerase II transcription subunit 25
Mediator complex subunit 25 family
ACID1; Activator Interaction Domain-Containing Protein 1; Activator-Recruited Cofactor 92 KDa Component; ARC/Mediator Transcriptional Coactivator Subunit; ARC92; BVSYS; CMT2B2; Mediator Complex Subunit 25; Mediator Of RNA Polymerase II Transcription Subunit 25; Mediator Of RNA Polymerase II Transcription, Subunit 25 Homolog (S. Cerevisiae); Mediator Of RNA Polymerase II Transcription, Subunit 25 Homolog; P78; TCBAP0758 MED25 ACID1, ARC92, BVSYS, CMT2B2, P78, PTOV2, TCBAP0758 mediator complex subunit 25 mediator of RNA polymerase II transcription subunit 25|ARC/mediator transcriptional coactivator subunit|activator interaction domain-containing protein 1|activator-recruited cofactor 92 kDa component|mediator of RNA polymerase II transcription, subunit 25 homolog|prostate-derived protein 78 kDa*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on MED25, check out the MED25 Infographic
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In this infographic, you will see the following information for MED25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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