Click image to see more details
Product Info Summary
|Description:||Transient overexpression lysate of multiple EGF-like-domains 10 (MEGF10)|
Customers Who Bought This Also Bought
MEGF10 (NM_032446) Human Over-expression Lysate
Transient overexpression lysate of multiple EGF-like-domains 10 (MEGF10)
Storage & Handling
Cite This Product
MEGF10 (NM_032446) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084466)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
Click image to see more details
MEGF10 Human Over-expression Lysates NM_032446.
Western blot validation of overexpression lysate (LS084466) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MEGF10 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for MEGF10 (Source: Uniprot.org, NCBI)
Multiple epidermal growth factor-like domains protein 10
Multiple epidermal growth factor-like domains protein 10 MEGF10 EMARDD, SR-F3 multiple EGF like domains 10 multiple epidermal growth factor-like domains protein 10*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on MEGF10, check out the MEGF10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MEGF10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for LS084466
Here are featured tools and databases that you might find useful:
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Have you used MEGF10 (NM_032446) Human Over-expression Lysate?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review MEGF10 (NM_032446) Human Over-expression Lysate
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question