MEK4 (MAP2K4) (NM_003010) Human Over-expression Lysate

Lysate of MKK4/MEK4 over-expression cell lines, WB control

Transient overexpression lysate of mitogen-activated protein kinase kinase 4 (MAP2K4)

Product Info Summary

SKU: LS006416
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of mitogen-activated protein kinase kinase 4 (MAP2K4)

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Product Name

MEK4 (MAP2K4) (NM_003010) Human Over-expression Lysate

See all MKK4/MEK4 products

SKU/Catalog Number

LS006416

Size

100 µg

Description

Transient overexpression lysate of mitogen-activated protein kinase kinase 4 (MAP2K4)

Storage & Handling

Cite This Product

MEK4 (MAP2K4) (NM_003010) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006416)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for MAP2K4 (Source: Uniprot.org, NCBI)

Uniprot ID

P45985

Gene ID

6416

Gene Name

MAP2K4

Full Name

Dual specificity mitogen-activated protein kinase kinase 4

Weight

44.288kDa

Superfamily

protein kinase superfamily

Alternative Names

c-Jun N-terminal kinase kinase 1; dual specificity mitogen-activated protein kinase kinase 4; JNK activating kinase 1; JNK-activated kinase 1; JNK-activating kinase 1; JNKK; JNKK1; JNKK1MAPK/ERK kinase 4; MAP kinase kinase 4; MAP2K4; MAPKK4; MEK4; MEK4MEK 4; mitogen-activated protein kinase kinase 4; MKK4; MKK4SAPK/ERK kinase 1; PRKMK4; PRKMK4MAPKK 4; SEK1; SERK1; SERK1EC 2.7.12.2 MAP2K4 JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1 mitogen-activated protein kinase kinase 4 dual specificity mitogen-activated protein kinase kinase 4|JNK-activated kinase 1|JNK-activating kinase 1|MAP kinase kinase 4|MAPK/ERK kinase 4|MAPKK 4|MEK 4|SAPK/ERK kinase 1|c-Jun N-terminal kinase kinase 1|stress-activated protein kinase kinase 1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on MAP2K4, check out the MAP2K4 Infographic

MAP2K4 infographic

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In this infographic, you will see the following information for MAP2K4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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