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Product Info Summary
|Description:||Transient overexpression lysate of family with sequence similarity 188, member B (FAM188B)|
MINDY4 (NM_032222) Human Over-expression Lysate
Transient overexpression lysate of family with sequence similarity 188, member B (FAM188B)
Storage & Handling
Cite This Product
MINDY4 (NM_032222) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084182)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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MINDY4 Human Over-expression Lysates NM_032222.
Western blot validation of overexpression lysate (LS084182) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with FAM188B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for MINDY4 (Source: Uniprot.org, NCBI)
Probable ubiquitin carboxyl-terminal hydrolase MINDY-4
MINDY deubiquitinase family
Probable ubiquitin carboxyl-terminal hydrolase MINDY-4 MINDY4 AQP-1, AQP1, C7orf67, CHIP28, FAM188B MINDY lysine 48 deubiquitinase 4 probable ubiquitin carboxyl-terminal hydrolase MINDY-4|Aquaporin-1|Aquaporin-CHIP|MINDY deubiquitinase 4|Urine water channel|Water channel protein for red blood cells and kidney proximal tubule|family with sequence similarity 188 member B|probable deubiquitinating enzyme MINDY-4|probable ubiquitin carboxyl-terminal hydrolase FAM188B|protein FAM188B*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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