MOGAT2 (NM_025098) Human Over-expression Lysate

Lysate of MOGAT2 over-expression cell lines, WB control

Transient overexpression lysate of monoacylglycerol O-acyltransferase 2 (MOGAT2)

Product Info Summary

SKU: LS080168
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of monoacylglycerol O-acyltransferase 2 (MOGAT2)

Product Name

MOGAT2 (NM_025098) Human Over-expression Lysate

See all MOGAT2 products

SKU/Catalog Number

LS080168

Size

100 µg

Description

Transient overexpression lysate of monoacylglycerol O-acyltransferase 2 (MOGAT2)

Storage & Handling

Cite This Product

MOGAT2 (NM_025098) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080168)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for MOGAT2 (Source: Uniprot.org, NCBI)

Gene Name

MOGAT2

Full Name

2-acylglycerol O-acyltransferase 2

Weight

38.196kDa

Superfamily

diacylglycerol acyltransferase family

Alternative Names

acyl CoA:monoacylglycerol acyltransferase 2; Acyl-CoA:monoacylglycerol acyltransferase 2; DC5; DGAT2L5MGC119183; Diacylglycerol acyltransferase 2-like protein 5; Diacylglycerol O-acyltransferase candidate 5; EC 2.3.1; EC 2.3.1.22; FLJ22644; hDC5; hMGAT2; MGAT2MGC119184; MGC119185; monoacylglycerol O-acyltransferase 22-acylglycerol O-acyltransferase 2 MOGAT2 DGAT2L5, DGAT2L5., MGAT2, hDC5 monoacylglycerol O-acyltransferase 2 2-acylglycerol O-acyltransferase 2|acyl-CoA:monoacylglycerol acyltransferase 2|diacylglycerol O-acyltransferase candidate 5|diacylglycerol acyltransferase 2-like protein 5

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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