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Product Info Summary
| SKU: | EK2380 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Monkey |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum, plasma(heparin, EDTA) |
Product info
Product Name
Monkey PD-L1 ELISA Kit PicoKine®
SKU/Catalog Number
EK2380
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Monkey PD-L1 ELISA Kit PicoKine® (96 Tests). Quantitate Monkey PD-L1 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Monkey PD-L1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2380)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: HEK293; Immunogen sequence: Met1-Thr239
Capture/Detection Antibodies
polyclonal antibody from goat|polyclonal antibody from goat
Sensitivity
<2 pg/mL
Assay Range
25.0-1,600 pg/mL
Standard Dilution Instructions
See datasheet of EK2380 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2380 is reactive to CD274 in Monkey samples
Validated Sample Types
cell culture supernatants, cell lysates, serum, plasma(heparin, EDTA)
Application Guarantee
EK2380 is guaranteed for ELISA in Monkey by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD274
Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a protein that in humans is encoded by the CD274 gene. Programmed death-ligand 1 (PD-L1) is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the adaptive arm of immune systems during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the adaptive immune system reacts to antigens that are associated with immune system activation by exogenous or endogenous danger signals.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2380-CAP | Anti-Monkey CD274 Pre-coated 96-well strip microplate | 1 |
| EK2380-ST | Monkey CD274 Standard | 2 vials, 10 ng/tube |
| EK2380-DA | Monkey CD274 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 25 | 50 | 100 | 200 | 400 | 800 | 1600 |
| O.D. | 0.006 | 0.03 | 0.065 | 0.123 | 0.254 | 0.613 | 1.283 | 1.957 |
Data Example Images
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Monkey PD-L1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Monkey PD-L1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 27 | 197 | 432 | 31 | 172 | 685 |
| Standard deviation | 0.87 | 11.09 | 32.27 | 2.24 | 4.76 | 28.56 |
| CV (%) | 3.2% | 5.6% | 7.5% | 7.2% | 2.8% | 4.2% |
Specific Publications For Monkey PD-L1 ELISA Kit PicoKine® (EK2380)
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