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Product Info Summary
| SKU: | EK0899-PR |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Monkey |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Monkey primate THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine®
View all Thrombospondin-1 ELISA kits
SKU/Catalog Number
EK0899-PR
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Monkey primate THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Monkey THBS1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Monkey primate THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0899-PR)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: N19-P1170
Sensitivity
<50 pg/ml
Assay Range
1.56 ng/ml - 100 ng/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0899-PR for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0899-PR is reactive to THBS1 in Monkey samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK0899-PR is guaranteed for ELISA in Monkey by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Thrombospondin-1
Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to monkey primate 15q15 and the cognate gene to monkey primate chromosome 2 (region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0899-PR-CAP | Anti-Monkey THBS1 Pre-coated 96-well strip microplate | 1 |
| EK0899-PR-ST | Monkey THBS1 Standard | 2 vials, 100 ng/tube |
| EK0899-PR-DA | Monkey THBS1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.116 | 0.225 | 0.297 | 0.401 | 0.747 | 1.194 | 1.693 | 2.174 |
Data Example Images
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Monkey Primate THBS1/TSP1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Monkey primate THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (ng/ml) | 15.5 | 32 | 52 | 16.5 | 38 | 64 |
| Standard deviation | 0.93 | 1.76 | 2.08 | 1.27 | 2.47 | 4.61 |
| CV (%) | 6 | 5.5 | 4 | 7.8 | 6.5 | 7.2 |
Protein Target Info & Infographic
Gene/Protein Information For THBS1 (Source: Uniprot.Org, NCBI)
Gene Name
THBS1
Full Name
Thrombospondin-1
Weight
129.383kDa
Superfamily
thrombospondin family
Alternative Names
THBS; THBS1; THBS-1; thrombospondin 1; Thrombospondin1; Thrombospondin-1; thrombospondin-1p180; TSP-1; TSP1thrombospondin-1; TSPTSP-1 THBS1 THBS, THBS-1, TSP, TSP-1, TSP1 thrombospondin 1 thrombospondin-1|glycoprotein G|thrombospondin-1p180|thrombospondin-p50
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on THBS1, check out the THBS1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for THBS1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Monkey primate THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine® (EK0899-PR)
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
6 Customer Q&As for Monkey primate THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for Thrombospondin-1 ELISA kit? I performed your Thrombospondin-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Thrombospondin-1 even though the O.D. values are not very high?
T. Thompson
Verified customer
Asked: 2020-03-07
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Thrombospondin-1 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Thrombospondin-1 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2020-03-07
Question
Q: if the enzyme conjugated Thrombospondin-1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Thrombospondin-1 antigen?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-12-05
Question
Q: how to analyze ELISA data? I have obtained Thrombospondin-1 level in serum.
L. Campbell
Verified customer
Asked: 2018-05-04
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-05-04
Question
Q: how can I thaw whole blood sample for Thrombospondin-1 ELISA after freezing?
T. Robinson
Verified customer
Asked: 2018-03-10
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Thrombospondin-1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-03-10
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
M. Thomas
Verified customer
Asked: 2016-10-10
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-10-10
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Thrombospondin-1 using the EK0899-PR Monkey Thrombospondin-1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-05-12
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2016-05-12
