|Product Name||Anti-SOCS1 Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Suppressor of cytokine signaling 1(SOCS1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-SOCS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1074)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human SOCS1(197-211aa NPVLRDYLSSFPFQI), identical to the related mouse and rat sequences.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-SOCS1 antibody, PA1074, Western blotting
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate
Anti-SOCS1 antibody, PA1074, IHC(P)
IHC(P): Rat Spleen Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Suppressor of cytokine signaling 1|
|Tissue Specificity||Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.|
|Alternative Names||Suppressor of cytokine signaling 1;SOCS-1;JAK-binding protein;JAB;STAT-induced STAT inhibitor 1;SSI-1;Tec-interacting protein 3;TIP-3;SOCS1;SSI1, TIP3;|
|Subcellular Localization||Nucleus . Cytoplasmic vesicle . Detected in perinuclear cytoplasmic vesicles upon interaction with FGFR3.|
|Molecular Weight||23551 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein- tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway. .|
|Research Areas||Adapters, Autoimmune, Cytoplasmic, Epigenetics And Nuclear Signaling, Immune System Diseases, Immunology, Nuclear Signaling Pathways, Signal Transduction
*You can search these to find other products in these research areas.
|Background||Suppressor of cytokine signaling 1(SOCS1), also known as Jak-binding protein(JAB), is a negative regulator to a subset of protein-tyrosine kinases. This 211-amino acid protein has a molecular mass of about 23.5 kD. The TEC-binding region of SOCS1 resides in the N terminus. SOCS1 associates with Tec and suppresses its kinase activity. The SOCS1 gene is intronless and is mapped to on chromosome 16p13.13. SOCS1 regulates the JAK/STAT signal-transduction pathway. Moreover, it contributes to protection against hepatic injury and fibrosis, and may also protect against hepatocarcinogenesis.|
Other Recommended Resources
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,