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Product Info Summary
| SKU: | EK1420 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®
View all Complement Factor D/Adipsin ELISA kits
SKU/Catalog Number
EK1420
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Adipsin / CFD / Factor D ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cfd in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1420)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: I26-S259
Sensitivity
<20 pg/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1420 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1420 is reactive to CFD in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1420 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Complement Factor D/Adipsin
Complement factor D (CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alternative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What’s more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1420-CAP | Anti-Mouse CFD Pre-coated 96-well strip microplate | 1 |
| EK1420-ST | Mouse CFD Standard | 2 vials, 50 ng/tube |
| EK1420-DA | Mouse CFD Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.131 | 0.211 | 0.242 | 0.355 | 0.535 | 0.889 | 1.466 | 2.017 |
Data Example Images
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Mouse CFD PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Complement Factor D/Adipsin, Dilution ratio of 1:20000, concentration in serum and plasma is 93-260 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1435 | 4423 | 19418 | 1328 | 4783 | 18803 |
| Standard deviation | 107.62 | 309.61 | 1378.67 | 111.55 | 411.33 | 1541.84 |
| CV (%) | 7.5% | 7% | 7.1% | 8.4% | 8.6% | 8.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Complement Factor D/Adipsin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1435 | 1482 | 1554 | 1340 | 1452 | 77.67 | 5.3% |
| Sample 2 | 4423 | 4063 | 4082 | 3952 | 4130 | 176.29 | 4.2% |
| Sample 3 | 19418 | 20714 | 18137 | 20289 | 19639 | 985.26 | 5% |
Protein Target Info & Infographic
Gene/Protein Information For CFD (Source: Uniprot.Org, NCBI)
Gene Name
CFD
Full Name
Complement factor D
Weight
27.033kDa
Superfamily
peptidase S1 family
Alternative Names
Adipsin; ADN; ADNcomplement factor D; AMBP-1; C3 convertase activator; CFD; complement factor D (adipsin); complement factor D preproprotein; Complement Factor D; D component of complement (adipsin); DF; EC 3.4.21; EC 3.4.21.46; PFD; Properdin factor DADIPSIN CFD ADIPSIN, ADN, DF, PFD complement factor D complement factor D|C3 convertase activator|D component of complement (adipsin)|complement factor D preproprotein|properdin factor D
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CFD, check out the CFD Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CFD: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine® (EK1420)
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No publications found for EK1420
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Customer Reviews
Have you used Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®?
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1 Reviews For Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®
0
Pretty good product with a reasonable price--Xiaobing Cui, University of Georgia, Physiology & Pharmacology, Research Scientist
Excellent
Source: Biocompare.com
| Application | ELISA |
|---|---|
| Sample | Serum and supernatant |
| Detection | -- |
"I did this assay twice. Both times were very good. First time I diluted the mouse serum and cell culture supernatant for 100-fold. However, the values of the serum are out of the range of standards. Then, I repeated the assay by diluting the mouse serum for 3000-fold. Much better than the 1st time, but still some values are marginally out of the range of standards. It is recommended that to do a dilution test using small amount of samples. Generally, you can get a very good result, just follow the instruction of this product. Easy to use. Good quality of data. It is a pretty good product with a reasonable price." (Customer’s Tip: "The serum needs to be diluted 5000-fold. The supernatant needs to be diluted 100-fold. Incubate for 10-15 min is enough after adding Color Developing Reagent.")
Xiaobing Cui
Verified customer
Submitted 2019-05-21
Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
11 Customer Q&As for Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®
Question
Does EK1420 contain sodium azide or thimerosal and 2-mercaptoethanol(or other toxic substances)?
Verified customer
Asked: 2022-06-04
Answer
The Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine® (EK1420) contains Proclin 300. Please see MSDS for detailed concentration in each component. https://www.bosterbio.com/msds?sku=EK1420
Boster Scientific Support
Answered: 2022-06-05
Question
Does EK1420 contain the protein derive from Baculovirus expression system?
Verified customer
Asked: 2022-06-04
Answer
The Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine® (EK1420) doesn't contain any protein derive from Baculovirus expression system.
Boster Scientific Support
Answered: 2022-06-05
Question
Was EK1420 purified by affinity column?
Verified customer
Asked: 2022-06-04
Answer
The antibody pair in the Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine® (EK1420) are Protein A or G purified.
Boster Scientific Support
Answered: 2022-06-05
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-09-18
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-09-18
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-04-06
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-03-27
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Complement Factor D using the EK1420 Mouse Complement Factor D Picokine® ELISA Kit?
R. Taylor
Verified customer
Asked: 2019-04-20
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2019-04-20
Question
Q: how to thaw whole blood sample for Complement Factor D ELISA after freezing?
Y. Thomas
Verified customer
Asked: 2019-02-09
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Complement Factor D for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-02-09
Question
Q: What is the optimal O.D. value for Complement Factor D ELISA kit? I performed your Complement Factor D ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Complement Factor D even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-08-27
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Complement Factor D ELISA kit to have sensitivity of 20pg/ml, that means the minimum amount of Complement Factor D that can be declared/interpreted as positive by the above standard is 20pg/ml.
Boster Scientific Support
Answered: 2018-08-27
Question
Q: Can Complement Factor D ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
A. Walker
Verified customer
Asked: 2018-04-10
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-04-10
Question
Q: how to proceed with the analysis of ELISA data? I have performed Complement Factor D level in serum.
V. Hill
Verified customer
Asked: 2017-12-21
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-12-21
