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Product Info Summary
| SKU: | PB9850 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Factor D/CFD Antibody Picoband®
SKU/Catalog Number
PB9850
PB0908 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Factor D/CFD Antibody Picoband® catalog # PB9850. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Factor D/CFD Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9850)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Factor D recombinant protein (Position: Q30-K235). Mouse Factor D shares 66.5% and 87.4% amino acid (aa) sequence identity with human and rat Factor D, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9850 is reactive to Cfd in Mouse
Observed Molecular Weight
43 kDa
Calculated molecular weight
28.1 kDa
Background of Cfd
Factor D is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene is a member of the trypsin family of peptidases. The encoded protein is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. It is also a serine protease that is secreted by adipocytes into the bloodstream. And it stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9850 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: mouse spleen tissue
Validation Images & Assay Conditions
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Western blot analysis of Factor D using anti-Factor D antibody (PB9850).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor D antigen affinity purified polyclonal antibody (Catalog # PB9850) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor D at approximately 43 kDa. The expected band size for Factor D is at 28 kDa.
Specific Publications For Anti-Factor D/CFD Antibody Picoband® (PB9850)
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1 Customer Q&As for Anti-Factor D/CFD Antibody Picoband®
Question
We are currently using anti-Factor D/CFD antibody PB9850 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on canine tissues as well?
D. Zhao
Verified customer
Asked: 2019-11-27
Answer
The anti-Factor D/CFD antibody (PB9850) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-27
