Mouse BMP-2 ELISA Kit PicoKine™

Mouse BMP-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Bmp2 in bone tissue, cell culture supernatants and serum. Sensitivity: 2pg/ml. Cited in 3 publication(s).

Product Info Summary

SKU: EK0313
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: bone tissue, cell culture supernatants and serum.

Product Name

Mouse BMP-2 ELISA Kit PicoKine™

See all Bmp2 products

SKU/Catalog Number

EK0313

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse BMP-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Bmp2 in bone tissue, cell culture supernatants and serum. Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse BMP-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0313)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q283-R396

Sensitivity

<2 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0313 is reactive to Bmp2 in Mouse samples

Validated Sample Types

bone tissue, cell culture supernatants and serum.

Application Guarantee

EK0313 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0313-CAP Anti-Mouse Bmp2 Pre-coated 96-well strip microplate 1
EK0313-ST Mouse Bmp2 Standard 2 vials, 10 ng/tube
EK0313-DA Mouse Bmp2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0020.0160.0270.0560.1790.4151.1182.016

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Bmp2, Dilution ratio of 1:1, concentration in serum is around 1ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse BMP-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)6224977061243710
Standard deviation2.5410.9558.522.6212.6355.38
CV (%)4.1%4.4%7.6%4.3%5.2%7.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Bmp2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 162706171664.526.8%
Sample 22492522462342456.832.7%
Sample 377085388185083841.354.9%
*number of samples for each test n=16.

Gene/Protein Information For Bmp2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P21274

Gene ID

12156

Gene Name

Bmp2

Full Name

Bone morphogenetic protein 2

Weight

44.525kDa

Superfamily

TGF-beta family

Alternative Names

BDA2; BMP2; BMP-2; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2; SSFSC Bmp2|AI467020, Bmpa, Bmp2|bone morphogenetic protein 2|bone morphogenetic protein 2|bone morphogenetic protein 2A

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Bmp2, check out the Bmp2 Infographic

Bmp2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Bmp2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0313 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhu Y,Liang H,Liu X,Wu J,Yang C,Wong TM, Kwan KYH,Cheung KMC,Wu S,Yeung KWK.Regulation of macrophage polarization through surface topography design to facilitate implant-to-bone osteointegration. Sci Adv.2021 Apr 2;7(14):eabf6654.doi:10. 1126/sciadv.abf6654.PMID:33811079.
Species: Mouse,Rat
EK0313 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA

Li-Bo Jiang, Sheng-Long Ding, Wang Ding, Di-Han Su, Fang-Xue Zhang, Tai-Wei Zhang, Xiao-Fan Yin, Lan Xiao, Yu-Lin Li, Feng-Lai Yuan, Jian Dong, Injectable Sericin Based Nanocomposite Hydrogel for Multi-modal Imaging-guided Immunomodulatory Bone Regeneration,Chemical Engineering Journal,2021,129323,ISSN 1385-8947,https://doi.org/10.1016/j.cej.2021.129323.
Species: Mouse,Rat
EK0313 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA

MicroRNA-218, microRNA-191*, microRNA-3070a and microRNA-33 are responsive to mechanical strain exerted on osteoblastic cells

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11 Customer Q&As for Mouse BMP-2 ELISA Kit PicoKine™

Question

Q: Can BMP2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

V. Lee

Verified customer

Asked: 2020-09-27

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-09-27

Question

Q: What is the optimal O.D. value for BMP2 ELISA kit? I performed your BMP2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain BMP2 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-05-29

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this BMP2 ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of BMP2 that can be declared/interpreted as positive by the above standard is 2pg/ml.

Boster Scientific Support

Answered: 2020-05-29

Question

Would the mouse antibody in this kit cross-react with the rat sample?

Verified Customer

Verified customer

Asked: 2020-03-24

Answer

BMP-2 is conservative and shows high homology in human, mouse and rat. Therefore, our mouse antibody in this kit should cross-react with the rat sample.

Boster Scientific Support

Answered: 2020-03-24

Question

Q: can you suggest the dilution ratio of serum samples for detection of BMP2 in Mouse bone tissue? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

S. Rodriguez

Verified customer

Asked: 2020-02-18

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the BMP2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-02-18

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

W. Evans

Verified customer

Asked: 2020-02-01

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-02-01

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-09-17

Question

Have you tested EK0313 with mouse plasma and what is the dilution ratio you used?

Verified customer

Asked: 2019-04-09

Answer

For the Mouse BMP-2 ELISA Kit PicoKine™ (EK0313), the suggested dilution ratio of mouse plasma is 1:1.

Boster Scientific Support

Answered: 2019-04-10

Question

Q: if the enzyme conjugated BMP2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the BMP2 antigen?

Verified Customer

Verified customer

Asked: 2019-01-06

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-01-06

Question

Q: how to thaw whole blood sample for BMP2 ELISA after freezing?

M. White

Verified customer

Asked: 2015-08-15

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test BMP2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2015-08-15

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

D. Phillips

Verified customer

Asked: 2015-03-14

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2015-03-14

Question

Hi, I am interested in your Mouse BMP-2 PicoKine

Verified Customer

Verified customer

Asked: 2014-03-12

Answer

The suggested dilution ratio of mouse plasma is 1:1.

Boster Scientific Support

Answered: 2014-03-12

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