Mouse CD10/Neprilysin ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Neprilysin/CD10. 96wells/kit, with removable strips.
Mouse CD10/Neprilysin ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Mouse CD10/Neprilysin ELISA Kit PicoKine™
See all Neprilysin/CD10 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Mouse Cd10/MME with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Cd10/MME tissue specificity:|
|Cite This Product||Mouse CD10/Neprilysin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0852)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: sf21; Immunogen sequence: Y52-W750|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For Mme (Source: Uniprot.org, NCBI)
|NCBI Gene Id||17380|
|Species Of This Entry||Mouse|
|Superfamily||peptidase M13 family|
|Alternative Names||Neprilysin/CD10|Atriopeptidase; CALLA; CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); CD10 antigen; CD10; CD10); CD10membrane metallo-endopeptidase variant 1; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24; EC 184.108.40.206; Enkephalinase; EPN; Leu-19; membrane metallo-endopeptidase variant 2; membrane metallo-endopeptidase; MGC126681; MGC126707; MME; NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA; Neprilysin; Neutral Endopeptidase 24.11; Neutral endopeptidase; NKH1; SFE; Skin fibroblast elastase|
|Post Tranlational Modifications||Cleavage; Methylation; Phosphorylation; Reduction; Hydroxylation; Glycosylation; Oxidation|
|Gene Location||3 E1|3 29.97 cM, on Chromasome 3, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Mme (Source: Uniprot.org, NCBI)
|Protein Function||Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) (By similarity). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).|
|Research Areas||Cancer; Cell Biology; Cytokine Research; Immunology; Stem Cell Markers|
|Related Diseases||Neoplasms; Malignant Neoplasms; Tissue Adhesions; Kidney Diseases; Neoplasm Metastasis; Carcinoma; Alzheimer's Disease; Tumor Angiogenesis; Depressive Disorder; Inflammation; Cell Transformation, Neoplastic; Pathologic Neovascularization; Malignant Neoplasm Of Prostate; Pain; Kidney Failure, Chronic; Mammary Neoplasms; Diabetes Mellitus; Colonic Neoplasms|
|Related Pathways||Secretion; Cell Cycle; Angiogenesis; Cell Adhesion; Cell Proliferation; Methylation; Localization; Mating; Aging; Proteolysis; Cell Division; Hatching; Pathogenesis; Inflammatory Response; Translation; Wound Healing; Cell Death; Cell Growth; Acute Inflammatory Response; Tropism|
|Background||Neprilysin, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), CD10, and common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease, this gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, Neprilysin is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. Neprilysin has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.|
|Scientific References||PMID: 1374101 by Chen C.Y., et al. Murine common acute lymphoblastic leukemia antigen (CD10 neutral endopeptidase 24.11). Molecular characterization, chromosomal localization, and modeling of the active site.|
PMID: 20876573 by Morisaki N., et al. Neprilysin is identical to skin fibroblast elastase: its role in skin aging and UV responses.
Kit Components And Assay QC Data Details Of Mouse CD10/Neprilysin ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0852-CAP||96-well plate precoated with anti-Mouse Neprilysin/CD10 antibody||1|
|EK0852-ST||lyophilized recombinant Mouse Neprilysin/CD10 standard||10ng/tube|
|EK0852-DA||biotinylated anti-Mouse Neprilysin/CD10 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0852 Mouse CD10/Neprilysin ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse CD10/Neprilysin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse CD10/Neprilysin ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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