Mouse CD10/Neprilysin ELISA Kit PicoKine™

Mouse CD10/Neprilysin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mme in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0852
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse CD10/Neprilysin ELISA Kit PicoKine™

See all Neprilysin/CD10 products

SKU/Catalog Number

EK0852

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CD10/Neprilysin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mme in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse CD10/Neprilysin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0852)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: sf21; Immunogen sequence: Y52-W750

Sensitivity

<10 pg/ml

Assay Range

93.7 pg/ml - 6,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0852 is reactive to Mme in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0852 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0852-CAP Anti-Mouse Mme Pre-coated 96-well strip microplate 1
EK0852-ST Mouse Mme Standard 2 vials, 10 ng/tube
EK0852-DA Mouse Mme Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)093.7187.53757501300030006000
O.D.0.1050.2090.2740.4210.7841.3581.9672.481

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Neprilysin/CD10, Dilution ratio of 1:1, concentration in serum is around 200pg/ml.

Some articles we found to cite concentrations of Neprilysin/CD10 in samples: 24972738 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse CD10/Neprilysin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)165102132281759513447
Standard deviation7.2641.86216.274.7%257.4%5255.07
CV (%)4.4%4.1%6.7%4.7%5.8%7.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Neprilysin/CD10 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11651781861771767.54.2%
Sample 210219079951027987484.8%
Sample 33228339433453463335785.712.5%
*number of samples for each test n=16.

Gene/Protein Information For Mme (Source: Uniprot.Org, NCBI)

Uniprot ID

Q61391

Gene ID

17380

Gene Name

Mme

Full Name

Neprilysin

Weight

85.702kDa

Superfamily

peptidase M13 family

Alternative Names

Atriopeptidase; CALLA; CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); CD10 antigen; CD10; CD10); CD10membrane metallo-endopeptidase variant 1; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24; EC 3.4.24.11; Enkephalinase; EPN; Leu-19; membrane metallo-endopeptidase variant 2; membrane metallo-endopeptidase; MGC126681; MGC126707; MME; NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA; Neprilysin; Neutral Endopeptidase 24.11; Neutral endopeptidase; NKH1; SFE; Skin fibroblast elastase Mme|6030454K05Rik, C85356, CALLA, CD10, N, NEP, SFE, ne|membrane metallo endopeptidase|neprilysin|atriopeptidase|common acute lymphoblastic leukemia antigen|enkephalinase|neutral endopeptidase 24.11|skin fibroblast elastase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Mme, check out the Mme Infographic

Mme infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Mme: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Mouse CD10/Neprilysin ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-08-27

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-08-27

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CD10 using the EK0852 Mouse CD10 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-07-24

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2020-07-24

Question

For EK0852, a customer is wondering if samples prepared with citrate will work with this kit? Has samples prepared with citrate been tested?

Verified Customer

Verified customer

Asked: 2020-03-25

Answer

Plasma (Citrate) hasn't been validated by our lab technicians. We suggest the customer run a pilot test for Plasma (Citrate).

Boster Scientific Support

Answered: 2020-03-25

Question

Q: how can I thaw whole blood sample for CD10 ELISA after freezing?

P. Jha

Verified customer

Asked: 2020-03-24

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD10 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-03-24

Question

Q: if the enzyme conjugated CD10 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD10 antigen?

Verified Customer

Verified customer

Asked: 2019-04-09

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-04-09

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-03-19

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-03-19

Question

Q: how to analyze ELISA data? I have obtained CD10 level in plasma.

H. Carter

Verified customer

Asked: 2018-01-12

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-01-12

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