Mouse Complement C5a ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Complement C5a. 96wells/kit, with removable strips. Cited in 1 publication(s).
Mouse Complement C5a ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Mouse Complement C5a ELISA Kit PicoKine™
See all C5 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Mouse C5A/C5 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. C5A/C5 tissue specificity:|
|Cite This Product||Mouse Complement C5a ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0987)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: N679-R755|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For C5 (Source: Uniprot.org, NCBI)
|Species Of This Entry||Mouse|
|Protein Name||Complement C5|
|Alternative Names||C5|C5; Complement Component C5/C5a|
|Post Tranlational Modifications||Cleavage; Methylation; Oxidation; Phosphorylation; Reduction; Hydroxylation; Glycosylation; Carboxylation; Decarboxylation; Deamination; Acetylation; Acylation; Nitration; Phosphorothioation; Biotinylation; Deacetylation; Demethylation; Citrullination; Bromination; Dephosphorylation|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For C5 (Source: Uniprot.org, NCBI)
|Protein Function||Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.|
|Related Diseases||Spinal Cord Injuries; Pain; Inflammation; Neoplasms; Hemolysis (disorder); Quadriplegia; Spinal Cord Diseases; Flexed Fetal Attitude; Malignant Neoplasms; Compression Of Spinal Cord; Infective Disorder; Fracture; Weakness; Radiculopathy; Arthritis; Neck Pain; Salmonella Infections; Stenosis; Spinal Injuries; Edema|
|Related Pathways||Complement Activation; Pathogenesis; Methylation; Localization; Inflammatory Response; Transport; Immune Response; Reflex; Secretion; Chemotaxis; Ossification; Virulence; Regeneration; Phagocytosis; Coagulation; Innervation; Hypersensitivity; Opsonization; Dna Methylation; Translation|
|Background||C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.|
|Scientific References||PMID: 2303408 by Wetsel R.A., et al. Deficiency of the murine fifth complement component (C5). A 2-base pair gene deletion in a 5'-exon.|
PMID: 2436653 by Wetsel R.A., et al. Primary structure of the fifth component of murine complement.
Kit Components And Assay QC Data Details Of Mouse Complement C5a ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0987-CAP||96-well plate precoated with anti-Mouse C5 antibody||1|
|EK0987-ST||lyophilized recombinant Mouse C5 standard||10ng/tube|
|EK0987-DA||biotinylated anti-Mouse C5 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0987 Mouse Complement C5a ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse Complement C5a ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse Complement C5a ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 1
|The involvement of C5a in the progression of experimental arthritis with Porphyromonas gingivalis infection in SKG mice||PubMed ID 30390695|
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