Mouse Complement C5a ELISA Kit PicoKine™

Mouse Complement C5a ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse C5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0987
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse Complement C5a ELISA Kit PicoKine™

See all C5 products

SKU/Catalog Number

EK0987

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Complement C5a ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse C5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse Complement C5a ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0987)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N679-R755

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0987 is reactive to C5 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0987 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0987-CAP Anti-Mouse C5 Pre-coated 96-well strip microplate 1
EK0987-ST Mouse C5 Standard 2 vials, 10 ng/tube
EK0987-DA Mouse C5 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0180.1030.1780.2440.5771.0451.5872.057

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect C5, Dilution ratio of 1:100, concentration in serum is around 10ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Complement C5a ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3822949935224532
Standard deviation1.7816.9438.922.118.8149.47
CV (%)4.7%7.4%7.8%6%8.4%9.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of C5 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 138403637371.473.9%
Sample 222923019120721416.267.5%
Sample 349952847746149125.145.1%
*number of samples for each test n=16.

Gene/Protein Information For C5 (Source: Uniprot.Org, NCBI)

Uniprot ID

P06684

Gene ID

Gene Name

C5

Full Name

Complement C5

Weight

188.878kDa

Alternative Names

C5; Complement Component C5/C5a

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on C5, check out the C5 Infographic

C5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for C5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0987 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The involvement of C5a in the progression of experimental arthritis with Porphyromonas gingivalis infection in SKG mice

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12 Customer Q&As for Mouse Complement C5a ELISA Kit PicoKine™

Question

Q: can I use citrate plasma as samples in Mouse C5 Picokine® ELISA Kit (Catalog # EK0987)?

Verified Customer

Verified customer

Asked: 2020-10-19

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of C5 causing disruption of its protein structure. C5 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the C5 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-10-19

Question

Q: What is the optimal O.D. value for C5 ELISA kit? I performed your C5 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain C5 even though the O.D. values are not very high?

B. Roberts

Verified customer

Asked: 2020-05-06

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this C5 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of C5 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2020-05-06

Question

Q: if the enzyme conjugated C5 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the C5 antigen?

Verified Customer

Verified customer

Asked: 2020-04-18

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-04-18

Question

Does the antibody in this kit react with C5?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

C5 precursor is first processed by the removal of four basic residues, forming two chains (alpha and beta), linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin. C5a is derived from proteolytic degradation of complement C5. Our C5a ELISA kit measures the natural and recombinant C5a (C5a) by itself or in the context of C5.

Boster Scientific Support

Answered: 2020-01-08

Question

What is the suggested dilution for mouse serum samples?

Verified Customer

Verified customer

Asked: 2019-12-09

Answer

Our suggested dilution for mouse serum when using EK0987 is 1:100 and the concentration range for normal serum is 6-20ng/mL.

Boster Scientific Support

Answered: 2019-12-09

Question

Q: Can C5 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

H. Green

Verified customer

Asked: 2019-10-28

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-10-28

Question

Does this kit recognize uncleaved C5?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

EK0987 is able to recognize the uncleaved C5.

Boster Scientific Support

Answered: 2019-07-25

Question

What is the suggested dilution ratio for EK0987 for plasma samples collected from heparin tubes?

Verified customer

Asked: 2019-06-12

Answer

The suggested dilution ratio for the Mouse Complement C5a ELISA Kit PicoKine™ (EK0987) plasma is 1:10-1:100.

Boster Scientific Support

Answered: 2019-06-13

Question

Q: how do I analyze ELISA data? I have performed C5 level in serum.

Verified Customer

Verified customer

Asked: 2018-11-22

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-11-22

Question

The product information states, “There is no detectable cross-reactivity with other relevant proteins.” Which relevant proteins were tested to have no cross-reactivity for EK0987?

Verified Customer

Verified customer

Asked: 2018-10-08

Answer

Our lab tested the following proteins and found no significant cross-reactivity or interference: <br> Recombinant mouse: Complement C3d <br> Recombinant human: Complement C3a Complement C5a

Boster Scientific Support

Answered: 2018-10-08

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-03-25

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-03-25

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2014-11-22

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2014-11-22

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