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Product Info Summary
| SKU: | EK0736 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma ( heparin). |
Product info
Product Name
Mouse CXCL10/IP-10 ELISA Kit PicoKine®
View all CXCL10/IP-10/CRG-2 ELISA kits
SKU/Catalog Number
EK0736
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CXCL10/IP-10 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cxcl10 in cell culture supernatants, serum and plasma ( heparin). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CXCL10/IP-10 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0736)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: I22-P98
Sensitivity
<1 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0736 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0736 is reactive to CXCL10 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma ( heparin).
Application Guarantee
EK0736 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CXCL10/IP-10/CRG-2
Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (gamma-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0736-CAP | Anti-Mouse CXCL10 Pre-coated 96-well strip microplate | 1 |
| EK0736-ST | Mouse CXCL10 Standard | 2 vials, 10 ng/tube |
| EK0736-DA | Mouse CXCL10 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.044 | 0.072 | 0.104 | 0.162 | 0.292 | 0.580 | 1.126 | 2.069 |
Data Example Images
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Mouse CXCL10/IP-10 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CXCL10/IP-10/CRG-2, Dilution ratio of 1:1, concentration in serum and plasma is 48-267 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CXCL10/IP-10 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 105 | 245 | 923 | 110 | 248 | 879 |
| Standard deviation | 10.237 | 13.72 | 47.99 | 10.23 | 18.6 | 56.25 |
| CV (%) | 7.5% | 5.6% | 5.2% | 9.3% | 7.5% | 6.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CXCL10/IP-10/CRG-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 105 | 98 | 104 | 102 | 102 | 2.68 | 2.6% |
| Sample 2 | 245 | 274 | 275 | 259 | 263 | 12.29 | 4.6% |
| Sample 3 | 923 | 908 | 845 | 862 | 884 | 32.01 | 3.6% |
Protein Target Info & Infographic
Gene/Protein Information For CXCL10 (Source: Uniprot.Org, NCBI)
Gene Name
CXCL10
Full Name
C-X-C motif chemokine 10
Weight
10.881kDa
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10 CXCL10 C7, IFI10, INP10, IP-10, SCYB10, crg-2, gIP-10, mob-1 C-X-C motif chemokine ligand 10 C-X-C motif chemokine 10|10 kDa interferon gamma-induced protein|gamma IP10|interferon-inducible cytokine IP-10|protein 10 from interferon (gamma)-induced cell line|small inducible cytokine subfamily B (Cys-X-Cys), member 10|small-inducible cytokine B10
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CXCL10, check out the CXCL10 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CXCL10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CXCL10/IP-10 ELISA Kit PicoKine® (EK0736)
Hello CJ!
EK0736 has been cited in 12 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Mixed-lineage kinase 3 pharmacological inhibition attenuates murine nonalcoholic steatohepatitis
Cryo-thermal therapy inducing MI macrophage polarization created CXCL10 and IL-6-rich pro-inflammatory environment for CD4+ T cell-mediated anti-tumor immunity
Antiviral effect of fufang yinhua jiedu (FFYH) granules against influenza A virus through regulating the inflammatory responses by TLR7/MyD88 signaling pathway
Activation of CXCL10/CXCR3 Signaling Attenuates Morphine Analgesia: Involvement of Gi Protein
Zhang Y,Wang R,Shi W,Zhihui Z,Wang X,Li C,Zhang S,Zhang P. Antiviral effect of fufang yinhua jiedu (FFYH) granules against influenza A virus through regulating the inflammatory responses by TLR7/MyD88 signaling pathway.J Ethnopharmacol.2021 Apr 1:114063.doi:10. 1016/j.jep.2021.114063.Epub ahead of print.PMID:33813013.
Species: Mouse,Canine
EK0736 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Regulation of TRIF-mediated innate immune response by K27-linked polyubiquitination and deubiquitination
The Zinc-Finger Protein ZCCHC3 Binds RNA and Facilitates Viral RNA Sensing and Activation of the RIG-I-like Receptors
ZCCHC3 is a co-sensor of cGAS for dsDNA recognition in innate immune response
USP44 positively regulates innate immune response to DNA viruses through deubiquitinating MITA
Activation of CXCL10/CXCR3 signaling attenuates morphine analgesia: involvement of Gi protein
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Mouse CXCL10/IP-10 ELISA Kit PicoKine®
Question
Q: how can I thaw whole blood sample for CXCL10 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-06-12
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CXCL10 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-06-12
Question
Q: if the enzyme conjugated CXCL10 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CXCL10 antigen?
Verified Customer
Verified customer
Asked: 2020-04-19
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-04-19
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
S. Babu
Verified customer
Asked: 2019-10-06
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-10-06
Question
Q: can I use EDTA plasma as samples in Mouse CXCL10 Picokine® ELISA Kit (Catalog # EK0736)?
Verified Customer
Verified customer
Asked: 2019-09-21
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CXCL10 causing disruption of its protein structure. CXCL10 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CXCL10 ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-09-21
Question
Q: how to proceed with the analysis of ELISA data? I have performed CXCL10 level in serum.
Verified Customer
Verified customer
Asked: 2019-06-23
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-06-23
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
E. Martin
Verified customer
Asked: 2016-10-14
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-10-14
