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Product Info Summary
| SKU: | FEK0326 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine |
| Assay Time: | 90 Minutes |
How Quick Picokine(R) ELISA kits work: Get data in 90 minutes
Product info
Product Name
Mouse EGF PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0326
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse EGF PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Egf in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 1 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Mouse EGF PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0326)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: N977-R1029
Capture/Detection Antibodies
The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<1 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Standard Dilution Instructions
See datasheet of FEK0326 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0326 is reactive to Egf in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine
Application Guarantee
FEK0326 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Egf
Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF acts by binding with high affinity to epidermal growth factor receptor(EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0326-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0326-ST | Mouse Egf Standard | 2 | 10 ng/tube |
| FEK0326-A | Mouse Egf Antibody A | 1 | 3ml |
| FEK0326-B | Mouse Egf Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.045 | 0.190 | 0.322 | 0.554 | 0.989 | 1.746 | 2.328 | 2.403 |
Data Example Images
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Human EGFR PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse EGF PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 18 | 52 | 277 | 19 | 63 | 274 |
| Standard deviation | 1.35 | 3.43 | 15.24 | 0.97 | 5.1 | 18.08 |
| CV(%) | 7.5% | 6.6% | 5.5% | 5.1% | 8.1% | 6.6% |
Protein Target Info & Infographic
Gene/Protein Information For Egf (Source: Uniprot.Org, NCBI)
Gene Name
Egf
Full Name
Weight
133.1 kDa
For more info on Egf, check out the Egf Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Egf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us support@bosterbio.com.
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