Mouse Fibronectin ELISA Kit PicoKine®

Fibronectin ELISA kit for Mouse

Mouse Fibronectin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Fn1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml. Cited in 16 publication(s).

Product Info Summary

SKU: EK0351
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Mouse Fibronectin ELISA Kit PicoKine®

View all Fibronectin ELISA kits

SKU/Catalog Number

EK0351

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Fibronectin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Fn1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse Fibronectin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0351)

Clonality of Antibodies

See Datasheet for details

Immunogen

Target protein isolated from mouse plasma

Sensitivity

<15 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0351 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0351 is reactive to FN1 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0351 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Fibronectin

Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.

Kit Components

Catalog Number Description Quantity
EK0351-CAP Anti-Mouse FN1 Pre-coated 96-well strip microplate 1
EK0351-ST Mouse FN1 Standard 2 vials, 10 ng/tube
EK0351-DA Mouse FN1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0480.0850.1790.2640.3660.7411.2581.990

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Fibronectin, Dilution ratio of 1:400000, concentration in serum and plasma is around 500 ug/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Fibronectin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2061683432321918433947
Standard deviation13.39126.22293.9615.54160.34335.49
CV (%)6.5%7.5%6.8%7.1%8.7%8.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Fibronectin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 120622923521522111.45.1%
Sample 21683159717491766169866.423.9%
Sample 343234412479640124385279.566.3%
*number of samples for each test n=16.

Gene/Protein Information For FN1 (Source: Uniprot.Org, NCBI)

Gene Name

FN1

Full Name

Fibronectin

Weight

272.32kDa

Alternative Names

CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF FN1 CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF fibronectin 1 fibronectin|cold-insoluble globulin|epididymis secretory sperm binding protein|migration-stimulating factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FN1, check out the FN1 Infographic

FN1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK0351 has been cited in 16 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

A Chinese Herbal Formula Ameliorates Pulmonary Fibrosis by Inhibiting Oxidative Stress via Upregulating Nrf2

RAGE Deletion Confers Renoprotection by Reducing Responsiveness to Transforming Growth Factor-β and Increasing Resistance to Apoptosis

ANRIL regulates production of extracellular matrix proteins and vasoactive factors in diabetic complications

Damage-associated molecular patterns (DAMPs) released after burn are associated with inflammation and monocyte activation

Tuning the interactions between chiral plasmonic films and living cells
Species: Mouse

A Chinese Herbal Formula Ameliorates Pulmonary Fibrosis by Inhibiting Oxidative Stress via Upregulating Nrf2

Damage-associated molecular patterns (DAMPs) released after burn are associated with inflammation and monocyte activation

Zhao X, Xu L, Sun M, Ma W, Wu X, Xu C, Kuang H. Nat Commun. 2017 Dec 8;8(1):2007. doi: 10.1038/s41467-017-02268-8. Tuning the interactions between chiral plasmonic films and living cells

Lv C, Wu C, Zhou Yh, Shao Y, Wang G, Wang Qy. Int J Endocrinol. 2014;2014:658589. Doi: 10.1155/2014/658589. Epub 2014 Oct 30. Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction Via Mtor/P70S6K/4E-Bp1 Pathway.

Gao L, Huang W, Li J. Mol Cell Biochem. 2013 Oct;382(1-2):185-91. Doi: 10.1007/S11010-013-1733-4. Epub 2013 Jun 26. Nox1 Abet Mesangial Fibrogenesis Via Inos Induction In Diabetes.

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12 Customer Q&As for Mouse Fibronectin ELISA Kit PicoKine®

Question

Q: Can Fibronectin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-04-22

Question

Q: can I use citrate plasma as samples in Mouse Fibronectin Picokine® ELISA Kit (Catalog # EK0351)?

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Fibronectin causing disruption of its protein structure. Fibronectin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Fibronectin ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-02-26

Question

Q: how do I analyze ELISA data? I measured Fibronectin level in plasma.

Verified Customer

Verified customer

Asked: 2019-08-01

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-08-01

Question

What is the immunogen for the capture antibody for EK0351?

Verified customer

Asked: 2019-05-15

Answer

For the Mouse Fibronectin ELISA Kit PicoKine™ (EK0351), the immunogen is natural protein from plasma.

Boster Scientific Support

Answered: 2019-05-15

Question

What are tested samples for EK0351?

Verified customer

Asked: 2019-05-15

Answer

The tested samples for the Mouse Fibronectin ELISA Kit PicoKine™ (EK0351) are mouse cell culture supernates, serum and plasma (heparin, EDTA, citrate).

Boster Scientific Support

Answered: 2019-05-15

Question

What is the standard source protein for EK0351?

Verified customer

Asked: 2019-05-15

Answer

The standard source protein for the Mouse Fibronectin ELISA Kit PicoKine™ (EK0351) is Natural protein from plasma.

Boster Scientific Support

Answered: 2019-05-15

Question

Q: if the enzyme conjugated Fibronectin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Fibronectin antigen?

M. Mitchell

Verified customer

Asked: 2019-04-25

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-04-25

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-03-26

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-03-26

Question

What type of fibronectin does EK0351 detect - cellular fibronectin, plasma fibronectin, or both? <br> Keywords: detection, fibronectin type

Verified Customer

Verified customer

Asked: 2019-02-12

Answer

EK0351 detects both types of fibronectin, cellular and plasma.

Boster Scientific Support

Answered: 2019-02-12

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-01-31

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-01-31

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Fibronectin using the EK0351 Mouse Fibronectin Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-03-02

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2017-03-02

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

S. Puri

Verified customer

Asked: 2014-03-13

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2014-03-13

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