Mouse GDNF ELISA Kit PicoKine™

Mouse GDNF ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Gdnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 5 publication(s). Independently reviewed in 1 review(s).

Product Info Summary

SKU: EK0935
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse GDNF ELISA Kit PicoKine™

See all Gdnf products

SKU/Catalog Number

EK0935

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse GDNF ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Gdnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse GDNF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0935)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard:NS0; Immunogen sequence: S78-I211

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0935 is reactive to Gdnf in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0935 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0935-CAP Anti-Mouse Gdnf Pre-coated 96-well strip microplate 1
EK0935-ST Mouse Gdnf Standard 2 vials, 10 ng/tube
EK0935-DA Mouse Gdnf Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0470.0690.0900.1640.3120.5771.0141.808

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse GDNF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)101227789101217855
Standard deviation7.579.357.537.679.1166.69
CV (%)7.5%4.1%7%7.6%4.2%7.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Gdnf in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1101101104971002.482.4%
Sample 22272042142272189.664.4%
Sample 378992879289185060.937.1%
*number of samples for each test n=16.

Gene/Protein Information For Gdnf (Source: Uniprot.Org, NCBI)

Uniprot ID

P48540

Gene ID

14573

Gene Name

Gdnf

Full Name

Glial cell line-derived neurotrophic factor

Weight

23.662kDa

Superfamily

TGF-beta family

Alternative Names

Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3 Gdnf|AI385739, ATF|glial cell line derived neurotrophic factor|glial cell line-derived neurotrophic factor|astrocyte-derived trophic factor|neurotrophic factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Gdnf, check out the Gdnf Infographic

Gdnf infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Gdnf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0935 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Granulocyte colony-stimulating factor promotes behavioral recovery in a mouse model of traumatic brain injury

Yang C, Zhou L, Gao X, Chen B, Tu J, Sun H, Liu X, He J, Liu J, Yuan Q. Neurosurgery. 2011 Mar;68(3):691-704. Doi: 10.1227/Neu.0B013E3182098A8A. Neuroprotective Effects Of Bone Marrow Stem Cells Overexpressing Glial Cell Line-Derived Neurotrophic ...

Liu G, Wang X, Shao G, Liu Q. Mol Med Rep. 2014 Apr;9(4):1305-12. Doi: 10.3892/Mmr.2014.1963. Epub 2014 Feb 18. Genetically Modified Schwann Cells Producing Glial Cell Line-Derived Neurotrophic Factor Inhibit Neuronal Apoptosis In Rat Spinal Cord ...

Chai L, Guo H, Li H, Wang S, Wang Yl, Shi F, Hu Lm, Liu Y, Adah D. J Ethnopharmacol. 2013 Oct 28;150(1):100-7. Doi: 10.1016/J.Jep.2013.08.011. Epub 2013 Sep 4. Scutellarin And Caffeic Acid Ester Fraction, Active Components Of Dengzhanxixin Injecti...

Banerjee A, N??rnberger S, Hennerbichler S, Riedl S, Schuh Cm, Hacobian A, Teuschl A, Eibl J, Redl H, Wolbank S. Cell Tissue Bank. 2014 Jun;15(2):227-39. Doi: 10.1007/S10561-013-9401-1. Epub 2013 Oct 29. In Toto Differentiation Of Human Amniotic M...

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1 Reviews For Mouse GDNF ELISA Kit PicoKine™

0

"Easy to Use GDNF ELISA Kit"

Excellent

Source: Biocompare.com

"Easy to Use GDNF ELISA Kit"

--Hannah Oakes, EAST TENNESSEE STATE UNIVERSITY, BIOMEDICAL SCIENCES, GRADUATE ASSISTANT

Applications Detecting GDNF
Sample Brain Tissue (Hippocampus)
Detection --

"We used this kit and the BDNF kit, because we were looking at neurogenesis within the hippocampus. We used the ELISA to complement our immunohistochemistry. It was easy to optimize the kits and the instructions were extremely easy to follow. Additionally, the instructions do not include using samples from tissue homogenates, but we did and the plates came out great. This product was selected after speaking with a representative about it at a conference. I am extremely glad we decided to go with this kit. This is a great kit. The protocol was easy to follow and optimize. We will be using these kits again."

Verified customer

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6 Customer Q&As for Mouse GDNF ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-04-14

Question

Would EK0935 work on tissue homogenate samples that has been prepared with RIPA buffer?

Verified customer

Asked: 2019-04-01

Answer

Mouse GDNF ELISA Kit PicoKine™ (EK0935) hasn't been tested on tissue homogenates. Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend to run a pilot test as described below to confirm the affect of endogeous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, proceed and work on the tissue sample by using the kit.

Boster Scientific Support

Answered: 2019-04-02

Question

Q: if the enzyme conjugated GDNF antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the GDNF antigen?

Verified Customer

Verified customer

Asked: 2018-05-21

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-05-21

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-03-30

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-03-30

Question

Q: how do I analyze ELISA data? I have performed GDNF level in plasma.

Verified Customer

Verified customer

Asked: 2015-03-27

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2015-03-27

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis GDNF using the EK0935 Mouse GDNF Picokine® ELISA Kit?

E. Liu

Verified customer

Asked: 2014-11-24

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2014-11-24

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