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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Mouse GDNF ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse GDNF ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Gdnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse GDNF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0935)
Clonality of Antibodies
See Datasheet for details
Expression system for standard:NS0; Immunogen sequence: S78-I211
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0935 is reactive to Gdnf in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0935 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Gdnf
Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures.2 GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.
|EK0935-CAP||Anti-Mouse Gdnf Pre-coated 96-well strip microplate||1|
|EK0935-ST||Mouse Gdnf Standard||2 vials, 10 ng/tube|
|EK0935-DA||Mouse Gdnf Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse GDNF PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse GDNF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Gdnf in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Gdnf (Source: Uniprot.Org, NCBI)
Glial cell line-derived neurotrophic factor
Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3 Gdnf|AI385739, ATF|glial cell line derived neurotrophic factor|glial cell line-derived neurotrophic factor|astrocyte-derived trophic factor|neurotrophic factor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Gdnf, check out the Gdnf Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Gdnf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0935 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Granulocyte colony-stimulating factor promotes behavioral recovery in a mouse model of traumatic brain injury
Yang C, Zhou L, Gao X, Chen B, Tu J, Sun H, Liu X, He J, Liu J, Yuan Q. Neurosurgery. 2011 Mar;68(3):691-704. Doi: 10.1227/Neu.0B013E3182098A8A. Neuroprotective Effects Of Bone Marrow Stem Cells Overexpressing Glial Cell Line-Derived Neurotrophic ...
Liu G, Wang X, Shao G, Liu Q. Mol Med Rep. 2014 Apr;9(4):1305-12. Doi: 10.3892/Mmr.2014.1963. Epub 2014 Feb 18. Genetically Modified Schwann Cells Producing Glial Cell Line-Derived Neurotrophic Factor Inhibit Neuronal Apoptosis In Rat Spinal Cord ...
Chai L, Guo H, Li H, Wang S, Wang Yl, Shi F, Hu Lm, Liu Y, Adah D. J Ethnopharmacol. 2013 Oct 28;150(1):100-7. Doi: 10.1016/J.Jep.2013.08.011. Epub 2013 Sep 4. Scutellarin And Caffeic Acid Ester Fraction, Active Components Of Dengzhanxixin Injecti...
Banerjee A, N??rnberger S, Hennerbichler S, Riedl S, Schuh Cm, Hacobian A, Teuschl A, Eibl J, Redl H, Wolbank S. Cell Tissue Bank. 2014 Jun;15(2):227-39. Doi: 10.1007/S10561-013-9401-1. Epub 2013 Oct 29. In Toto Differentiation Of Human Amniotic M...
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