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Product Info Summary
| SKU: | EK2070 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse GFRA1 ELISA Kit PicoKine®
View all GFR alpha-1/GDNF R alpha-1 ELISA kits
SKU/Catalog Number
EK2070
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse GFRA1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Gfra1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse GFRA1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2070)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: D25-S430
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2070 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2070 is reactive to GFRA1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2070 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of GFR alpha-1/GDNF R alpha-1
GDNF family receptor alpha-1 (GFRα1), also known as the GDNF receptor, is a protein that in humans is encoded by the GFRA1 gene. It is mapped to 19 D2; 19 54.18 cM. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.GDNF family receptor alpha 1 has been shown to interact with GDNF and RET proto-oncogene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2070-CAP | Anti-Mouse GFRA1 Pre-coated 96-well strip microplate | 1 |
| EK2070-ST | Mouse GFRA1 Standard | 2 vials, 10 ng/tube |
| EK2070-DA | Mouse GFRA1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.039 | 0.130 | 0.242 | 0.507 | 0.824 | 1.311 | 1.776 | 2.094 |
Data Example Images
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Mouse GFRA1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect GFR alpha-1/GDNF R alpha-1, Dilution ratio of 1:1, concentration in serum and plasma is around 227 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse GFRA1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 78 | 249 | 857 | 81 | 254 | 955 |
| Standard deviation | 3.20 | 11.45 | 45.42 | 4.46 | 17.02 | 71.63 |
| CV (%) | 4.1% | 4.6% | 5.3% | 5.5% | 6.7% | 7.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of GFR alpha-1/GDNF R alpha-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 17 | 16 | 16 | 16 | 16 | 0.43 | 2.6% |
| Sample 2 | 89 | 96 | 104 | 99 | 97 | 5.43 | 5.5% |
| Sample 3 | 450 | 497 | 503 | 495 | 486 | 21.13 | 4.3% |
Protein Target Info & Infographic
Gene/Protein Information For GFRA1 (Source: Uniprot.Org, NCBI)
Gene Name
GFRA1
Full Name
GDNF family receptor alpha-1
Weight
51.456kDa
Superfamily
GDNFR family
Alternative Names
DKFZp313E1012; DKFZp686J0156; GDNF family receptor alpha 1; GDNF family receptor alpha-1; GDNF R alpha-1; GDNF receptor alpha-1; GDNFRAGFR-ALPHA-1; GDNFR-alpha-1; GDNFRMGC23045; GFR alpha1; GFR alpha-1; GFRA1; GFRa-1; GFR-alpha-1; Glial cell line-derived neurotrophic factor receptor alpha; GPI-linked anchor protein; PI-linked cell-surface accessory protein; RET ligand 1; RET1L; RETL1FLJ10561; TGF-beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1; TRNR1FLJ31546 GFRA1 GDNFR, GDNFRA, GFR-ALPHA-1, GFRalpha-1, RET1L, RETL1, TRNR1 GDNF family receptor alpha 1 GDNF family receptor alpha-1|GDNF receptor alpha 1d|GDNF receptor alpha 1e|GDNFR-alpha-1|GPI-linked anchor protein|Glial cell line-derived neurotrophic factor receptor alpha|PI-linked cell-surface accessory protein|RET ligand 1|TGF-beta-related neurotrophic factor receptor 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GFRA1, check out the GFRA1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GFRA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse GFRA1 ELISA Kit PicoKine® (EK2070)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Mouse GFRA1 ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated GFR alpha-1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the GFR alpha-1 antigen?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-12-26
Question
Q: how to proceed with the analysis of ELISA data? I have obtained GFR alpha-1 level in serum.
Verified Customer
Verified customer
Asked: 2019-12-08
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-12-08
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification GFR alpha-1 using the EK2070 Mouse GFR alpha-1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-04-28
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-04-28
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
D. Walker
Verified customer
Asked: 2019-02-18
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-02-18
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-01-01
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-01-01
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
W. Dayal
Verified customer
Asked: 2018-10-04
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-10-04
