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Mouse Gp130/IL6ST ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Gp130/IL6ST. 96wells/kit, with removable strips.
Mouse Gp130/IL6ST ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0577 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Mouse |
| Sample Type: | cell culture supernates, serum and plasma(heparin, EDTA, citrate). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <10pg/ml |
| Assay Range: | 125pg/ml-8000pg/ml |
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Overview
| Product Name | Mouse Gp130/IL6ST ELISA Kit PicoKine™
See all Il6st primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0577 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Mouse Gp130/IL6ST with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Gp130/IL6ST tissue specificity: Found in tissues such as brain, heart, thymus, spleen, kidney, lung and liver. Found in all the cell lines tested except BaF-B03. Expression not restricted to IL6-responsive cells. |
| Cite This Product | Mouse Gp130/IL6ST ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0577) |
Product Specs
| Reactivity/Species | Mouse |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, serum and plasma(heparin, EDTA, citrate). |
| Sensitivity | <10pg/ml |
| Assay Range | 125pg/ml-8000pg/ml |
| Immunogen | Expression system for standard: NSO; Immunogen sequence: Q23-E617 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | Q00560 |
|---|---|
| Gene Name | Il6st |
| Protein Name | Interleukin-6 receptor subunit beta |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development. |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Mouse Gp130/IL6ST ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0577-CAP | 96-well plate precoated with anti-Mouse Il6st antibody | 1 |
| EK0577-ST | lyophilized recombinant Mouse Il6st standard | 10ng/tube |
| EK0577-DA | biotinylated anti-Mouse Il6st antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0577 Mouse Gp130/IL6ST ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
| O.D. | 0.018 | 0.080 | 0.153 | 0.284 | 0.483 | 0.884 | 1.445 | 2.148 |
We measured random samples of Mouse Gp130/IL6ST ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 159 | 1404 | 4588 | 158 | 1473 | 4382 |
| Standard deviation | 11.6 | 71.6 | 339.51 | 14.06 | 81.01 | 333.03 |
| CV(%) | 7.3% | 7.6% | 7.4% | 8.9% | 5.5% | 7.6% |
We measured the performance consistency of Mouse Gp130/IL6ST ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 159 | 185 | 185 | 168 | 174 | 11.21 | 6.4% |
| Sample 2 | 1404 | 1352 | 1232 | 1273 | 1315 | 66.97 | 5% |
| Sample 3 | 4588 | 4716 | 4423 | 4674 | 4600 | 112.25 | 2.4% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
Contact Information
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| (888) 466-3604 |
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All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.
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