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Product Info Summary
| SKU: | EK1217 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (EDTA, citrate). |
Product info
Product Name
Mouse HGF/Hepatocyte growth factor ELISA Kit PicoKine®
SKU/Catalog Number
EK1217
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse HGF/Hepatocyte growth factor ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Hgf in cell culture supernatants, serum and plasma (EDTA, citrate). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse HGF/Hepatocyte growth factor ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1217)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q33-R495 (α) & V496-L728 (β)
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1217 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1217 is reactive to HGF in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA, citrate).
Application Guarantee
EK1217 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of HGF
Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1217-CAP | Anti-Mouse HGF Pre-coated 96-well strip microplate | 1 |
| EK1217-ST | Mouse HGF Standard | 2 vials, 10 ng/tube |
| EK1217-DA | Mouse HGF Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.055 | 0.091 | 0.132 | 0.226 | 0.402 | 0.630 | 1.231 | 1.960 |
Data Example Images
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Mouse HGF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect HGF, For serum, dilution ratio is 1:5 and concentration is 5-8 ng/ml.
For plasma, dilution ratio is 1:10 and concentration is 4304-25422 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse HGF/Hepatocyte growth factor ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 249 | 1468 | 5963 | 259 | 1383 | 5909 |
| Standard deviation | 16.18 | 89.54 | 298.15 | 20.72 | 106.49 | 372.26 |
| CV (%) | 6.5% | 6.1% | 5% | 8% | 7.7% | 6.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of HGF in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 249 | 248 | 247 | 243 | 246 | 2.27 | 0.9% |
| Sample 2 | 1468 | 1414 | 1310 | 1392 | 1396 | 56.83 | 4% |
| Sample 3 | 5963 | 5251 | 5451 | 5032 | 5424 | 344.54 | 6.3% |
Protein Target Info & Infographic
Gene/Protein Information For HGF (Source: Uniprot.Org, NCBI)
Gene Name
HGF
Full Name
Hepatocyte growth factor
Weight
83.134kDa
Superfamily
peptidase S1 family
Alternative Names
deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A HGF DFNB39, F-TCFB, HPTA, SF, HGF hepatocyte growth factor hepatocyte growth factor|fibroblast-derived tumor cytotoxic factor|hepatocyte growth factor (hepapoietin A; scatter factor)|hepatopoietin-A|lung fibroblast-derived mitogen
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on HGF, check out the HGF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for HGF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse HGF/Hepatocyte growth factor ELISA Kit PicoKine® (EK1217)
Hello CJ!
EK1217 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Assessment of the involvement of the macrophage migration inhibitory factor%u2013glucocorticoid regulatory dyad in the expression of matrix metalloproteinase%u20102 during %u2026
Secchiero P, Corallini F, Zavan B, Tripodo C, Vindigni V, Zauli G. Invest New Drugs. 2012 Apr;30(2):803-7. Doi: 10.1007/S10637-010-9534-Z. Epub 2010 Sep 9. Mesenchymal Stem Cells Display Hepato-Protective Activity In Lymphoma Bearing Xenografts.
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Mouse HGF/Hepatocyte growth factor ELISA Kit PicoKine®
Question
Does EK1217 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Mouse HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK1217) doesn't contain native human biological materials. doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: if the enzyme conjugated HGF antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the HGF antigen?
Verified Customer
Verified customer
Asked: 2020-05-27
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-05-27
Question
Q: What is the optimal O.D. value for HGF ELISA kit? I used your HGF ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain HGF even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this HGF ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of HGF that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-02-19
Question
How much concentration of sodium azide or thimerosal and 2-mercaptoethanol (or other toxic substances) if it is contained in EK1217?
Verified customer
Asked: 2019-05-30
Answer
The Mouse HGF ELISA Kit PicoKine™ (EK1217) contains Sodium Azide 0.02mg/vial | 0.02%.
Boster Scientific Support
Answered: 2019-05-30
Question
Was EK1217 produced with Baculovirus to follow Cartagena protocol regulation?
Verified customer
Asked: 2019-05-30
Answer
The Mouse HGF ELISA Kit PicoKine™ (EK1217) was not produced with Baculovirus.
Boster Scientific Support
Answered: 2019-05-30
Question
Does EK1217 contain Stop Solution? What is its concentration (%) and ingredients?
Verified customer
Asked: 2019-05-30
Answer
The Mouse HGF ELISA Kit PicoKine™ (EK1217) contains a stop solution, Sulphuric acid 2N. Ingredient is just 2N sulfuric acid.
Boster Scientific Support
Answered: 2019-05-30
Question
Could you confirm if EK1217 is your manufactured item or not?
Verified customer
Asked: 2019-05-22
Answer
Yes, the Mouse HGF ELISA Kit PicoKine™ (EK1217) is from our lab.
Boster Scientific Support
Answered: 2019-05-22
Question
Q: can I use citrate plasma as samples in Mouse HGF Picokine® ELISA Kit (Catalog # EK1217)?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of HGF causing disruption of its protein structure. HGF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the HGF ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-05-16
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
D. Li
Verified customer
Asked: 2015-12-29
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2015-12-29
