Mouse IGFBP-3 ELISA Kit PicoKine™

Mouse IGFBP-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse IGFBP3 in cell culture supernatants and serum. Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0387
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Mouse IGFBP-3 ELISA Kit PicoKine™

See all IGFBP-3 products

SKU/Catalog Number

EK0387

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IGFBP-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse IGFBP3 in cell culture supernatants and serum. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse IGFBP-3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0387)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: G28Q-K291

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0387 is reactive to IGFBP3 in Mouse samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK0387 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0387-CAP Anti-Mouse IGFBP3 Pre-coated 96-well strip microplate 1
EK0387-ST Mouse IGFBP3 Standard 2 vials, 10 ng/tube
EK0387-DA Mouse IGFBP3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)0156312.562512500025000500010000
O.D.0.0330.0810.1110.1870.3170.7351.2512.029

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IGFBP-3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2691078549926610165861
Standard deviation13.9855.8%1335.4315.4261.97380.96
CV (%)5.2%5.4%6.1%5.8%6.1%6.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IGFBP-3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 126924622423824416.316.6%
Sample 21078124712091042114486.017.5%
Sample 354995733582151215543270.834.8%
*number of samples for each test n=16.

Gene/Protein Information For IGFBP3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P17936

Gene ID

3486

Gene Name

IGFBP3

Full Name

Insulin-like growth factor-binding protein 3

Weight

31.674kDa

Alternative Names

acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein; IBP-3; IBP3BP-53; IGF-binding protein 3; IGFBP3; IGFBP-3; insulin-like growth factor binding protein 3; insulin-like growth factor-binding protein 3 IGFBP3 BP-53, IBP3 insulin like growth factor binding protein 3 insulin-like growth factor-binding protein 3|IBP-3|IGF-binding protein 3|IGFBP-3|acid stable subunit of the 140 K IGF complex|binding protein 29|binding protein 53|growth hormone-dependent binding protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IGFBP3, check out the IGFBP3 Infographic

IGFBP3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IGFBP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0387 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

IGF-IR signaling attenuates the age-related decline of diastolic cardiac function

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5 Customer Q&As for Mouse IGFBP-3 ELISA Kit PicoKine™

Question

Hello Support; I wat to know if EK0387 Mouse IGFBP-3 ELISA Kit will detect both bound and unbound IGFBP3.

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

Unfortunately our EK0387 Mouse IGFBP-3 ELISA Kit is not yet tested this interaction, so we cannot give you any corresponding data. The EK0387 Mouse IGFBP-3 ELISA Kit is only for binding by the assay in which the free form is obviously recognized but the bound might or might not be recognized. Since, we do not map the location of the antibody epitopes, we do not know at what percentage, if any, of the bound form can be recognized.

Boster Scientific Support

Answered: 2020-04-09

Question

Q: how can I thaw whole blood sample for IGFBP-3 ELISA after freezing?

W. Dayal

Verified customer

Asked: 2019-09-09

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IGFBP-3 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-09-09

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-12-27

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-12-27

Question

Q: Can IGFBP-3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2015-11-03

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2015-11-03

Question

Q: how to proceed with the analysis of ELISA data? I have obtained IGFBP-3 level in serum.

Verified Customer

Verified customer

Asked: 2014-09-01

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2014-09-01

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