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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
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Mouse IL-12(p40) ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse IL-12 (p40) ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il12b in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse IL-12(p40) ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0932)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: sf21; Immunogen sequence: M23-S335
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0932 is reactive to Il12b in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
EK0932 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0932-CAP||Anti-Mouse Il12b Pre-coated 96-well strip microplate||1|
|EK0932-ST||Mouse Il12b Standard||2 vials, 10 ng/tube|
|EK0932-DA||Mouse Il12b Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse IL-12(p40) PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Il12b, Dilution ratio of 1:1, concentration in serum is around 300pg/ml.
Some articles we found to cite concentrations of Il12b in samples: 17513774 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-12(p40) ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Il12b in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Il12b (Source: Uniprot.Org, NCBI)
Interleukin-12 subunit beta
CLMF p40; CLMF; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 p40; IL-12 subunit p40; IL12B; IL-12B; IL-12BNK cell stimulatory factor chain 2; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NKSF; NKSF2; NKSF2IL12, subunit p40 Il12b|Il-1, Il-12, Il-12b, Il-12p40, Il12p40, p40|interleukin 12b|interleukin-12 subunit beta|CLMF p40|IL-12 p40 subunit|IL-12 subunit p40|IL-23 subunit p40|cytotoxic lymphocyte maturation factor 40 kDa subunit|interleukin-12 p40 subunit*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Il12b, check out the Il12b Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il12b: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0932 has been cited in 14 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
He Y, Peng H, Zhang H, Liu Y, Sun H. Structural characteristics and immunopotentiation activity of two polysaccharides from the petal of Crocus sativus. Int J Biol Macromol. 2021 Mar 4:S0141-8130(21)00523-7. doi: 10.1016/j.ijbiomac.2021.03. 006. Epub ahead of print. PMID: 33676979.
EK0932 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA
The dendritic cell mannose receptor mediates allergen internalization and maturation involving notch 1 signalling
Fusion cytokine IL-2-GMCSF enhances anticancer immune responses through promoting cell-cell interactions
Stimulation of dendritic cells by DAMPs in ALA-PDT treated SCC tumor cells
Li J, Diao B, Guo S, Huang X, Yang C, Feng Z, Yan W, Ning Q, Zheng L, Chen Y, Wu Y. Nat Commun. 2017 Nov 6;8(1):1322. doi: 10.1038/s41467-017-01327-4. VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate met...
Zhang A, Yang X, Li Q, Yang Y, Zhao G, Wang B, Wu D. PLoS One. 2018 Jan 23;13(1):e0191356. doi: 10.1371/journal.pone.0191356. eCollection 2018. Immunostimulatory activity of water-extractable polysaccharides from Cistanche deserticola as a plant a...
Du, J., Chen, X., Wang, C., & Sun, H. (2017). Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha. Journal of Ethnopharmacology. Advance online publication. doi: 1...
Li Y, Li X, Liu H, Zhuang S, Yang J, Zhang F. Oncol Lett. 2014 Feb;7(2):576-582. Epub 2013 Dec 9. Intranasal Immunization With Recombinant Lactococci Carrying Human Papillomavirus E7 Protein And Mouse Interleukin-12 Dna Induces E7-Specific Antitum...
Hara T, Mimura K, Abe T, Shioi G, Seiki M, Sakamoto T. J Biol Chem. 2011 Sep 16;286(37):32542-51. Doi: 10.1074/Jbc.M111.271726. Epub 2011 Jul 21. Deletion Of The Mint3/Apba3 Gene In Mice Abrogates Macrophage Functions And Increases Resistance To L...
Ma Yj, He M, Han Ja, Yang L, Ji Xy. Scand J Immunol. 2013 Oct;78(4):387-93. Doi: 10.1111/Sji.12097. A Clinical Study Of Hbsag-Activated Dendritic Cells And Cytokine-Induced Killer Cells During The Treatment For Chronic Hepatitis B.
Li Y, Li Sy, Li Js, Deng L, Tian Yg, Jiang Sl, Wang Y, Wang Yy. Biol Pharm Bull. 2012;35(10):1752-60. Epub 2012 Aug 3. A Rat Model For Stable Chronic Obstructive Pulmonary Disease Induced By Cigarette Smoke Inhalation And Repetitive Bacterial Infe...
Li J, Li Ff, Zuo W, Zhou Y, Hao Hy, Dang J, Jiang M, He Mz, Deng Dr. J Huazhong Univ Sci Technolog Med Sci. 2014 Aug;34(4):586-90. Doi: 10.1007/S11596-014-1320-2. Epub 2014 Aug 19. Up-Regulated Expression Of Tim-3/Gal-9 At Maternal-Fetal Interface...
Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (????????????
Pan Zy, Wang H. Int Immunopharmacol. 2014 May;20(1):229-37. Doi: 10.1016/J.Intimp.2014.03.004. Epub 2014 Mar 19. Synergistic Interaction Between Choline And Aspirin Against Acute Inflammation Induced By Carrageenan And Lipopolysaccharide.
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