Product Info Summary
SKU: | EZ0422 |
---|---|
Size: | For 5 plates, 96 wells each, reagents only, plates are not included |
Reactive Species: | Mouse |
Application: | ELISA |
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Product info
Product Name
Mouse IL-12(p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs)
SKU/Catalog Number
EZ0422
Size
For 5 plates, 96 wells each, reagents only, plates are not included
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-12(p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse IL-12(p70) in cell culture supernatants and serum.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IL-12(p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0422)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: (M23-S335) + (R23-A215)
Assay Range
15.6 pg/ml - 1,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EZ0422 is reactive to Il12a in Mouse samples
Validated Sample Types
cell culture supernatants and serum
Application Guarantee
EZ0422 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Catalog number | Description | Quantity | Storage of opened/reconstituted material |
---|---|---|---|
EZ0422-CA | Mouse anti- mouse IL-12(p70) monoclonal antibody (Capture Antibody) | 500 µl, 4 mg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
EZ0422-DA | Biotinylated goat anti- mouse IL-12(p70) polyclonal antibody (Detection Antibody) | 500 µl, 0.3 µg/mL (recommended dilution 1:100) | |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) | |
EK0422-ST | Lyophilized recombinant Mouse anti- mouse IL-12(p70) monoclonal standard | 10 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Incubator.
- 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 5. Clean tubes and Eppendorf tubes.
- 6. 96 well microplate (Cat# AR1100)
- 7. Plate Sealers.
- 8. Capture Antibody Diluent: PBS.
- 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. - *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.041 | 0.101 | 0.159 | 0.270 | 0.459 | 0.869 | 1.507 | 2.092 |
Data Example Images
Click image to see more details
Mouse IL-12 (p70) EZ Set ELISA Kit standard curve
Protein Target Info & Infographic
Gene/Protein Information For Il12a (Source: Uniprot.Org, NCBI)
Gene Name
Il12a
Full Name
Interleukin-12 subunit alpha
Weight
24.179kDa
Superfamily
IL-6 superfamily
Alternative Names
CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL12 p70; IL-12 p70; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35 Il12a|IL-12, IL-12p35, Il-12a, Ll12a, p3, p35|interleukin 12a|interleukin-12 subunit alpha|CLMF p35|IL-12 p35 subunit|IL-12 subunit p35|cytotoxic lymphocyte maturation factor 35 kDa subunit|interleukin 12a p35 subunit|interleukin-12 p35 subunit
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Il12a, check out the Il12a Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il12a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse IL-12(p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0422)
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