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Product Info Summary
| SKU: | EK0422 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Mouse IL-12(p70) ELISA Kit PicoKine®
SKU/Catalog Number
EK0422
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-12 (p70) ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse IL-12(p70) in cell culture supernatants and serum. Sensitivity: 1.5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IL-12(p70) ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0422)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: (M23-S335)+ (R23-A215)
Sensitivity
<1.5 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0422 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0422 is reactive to Il12a in Mouse samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0422 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Il12a
Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0422-CAP | Anti-Mouse Il12a Pre-coated 96-well strip microplate | 1 |
| EK0422-ST | Mouse Il12a Standard | 2 vials, 10 ng/tube |
| EK0422-DA | Mouse Il12a Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.041 | 0.101 | 0.159 | 0.270 | 0.459 | 0.869 | 1.507 | 2.092 |
Data Example Images
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Mouse IL-12(p70) PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Il12a, Dilution ratio of 1:1, concentration in serum is 2-40 pg/ml..
Some articles we found to cite concentrations of Il12a in samples: 17430656 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-12(p70) ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 37 | 150 | 410 | 33 | 147 | 442 |
| Standard deviation | 2.18 | 9.15 | 30.75 | 2.44 | 8.96 | 38.45 |
| CV (%) | 7.5% | 6.1% | 7.5% | 7.4% | 6.1% | 8.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Il12a in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 37 | 38 | 39 | 40 | 38 | 1.11 | 2.9% |
| Sample 2 | 150 | 151 | 171 | 168 | 160 | 9.56 | 5.9% |
| Sample 3 | 410 | 416 | 442 | 417 | 421 | 12.27 | 2.9% |
Protein Target Info & Infographic
Gene/Protein Information For Il12a (Source: Uniprot.Org, NCBI)
Gene Name
Il12a
Full Name
Interleukin-12 subunit alpha
Weight
24.179kDa
Superfamily
IL-6 superfamily
Alternative Names
CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL12 p70; IL-12 p70; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35 Il12a|IL-12, IL-12p35, Il-12a, Ll12a, p3, p35|interleukin 12a|interleukin-12 subunit alpha|CLMF p35|IL-12 p35 subunit|IL-12 subunit p35|cytotoxic lymphocyte maturation factor 35 kDa subunit|interleukin 12a p35 subunit|interleukin-12 p35 subunit
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Il12a, check out the Il12a Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il12a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse IL-12(p70) ELISA Kit PicoKine® (EK0422)
Hello CJ!
EK0422 has been cited in 48 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Upregulation of Integrin αDβ2 (CD11d/CD18) on Inflammatory Macrophages Promotes Macrophage Retention in Vascular Lesions and Development of Atherosclerosis
Intranasal immunization with recombinant Lactococci carrying human papillomavirus E7 protein and mouse interleukin‑12 DNA induces E7‑specific antitumor effects in C57BL/6 mice
H9N2 Avian Influenza Virus Protein PB1 Enhances the Immune Responses of Bone Marrow-Derived Dendritic Cells by Down-Regulating miR375
MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells via down-regulated Mbnl3
4,4′-diaponeurosporene, a C30 carotenoid, effectively activates dendritic cells via CD36 and NF-κB signaling in a ROS independent manner
Exposure to Low-Dose Radiation Enhanced the Antitumor Effect of a Dendritic Cell Vaccine:
Acetylation Modification Improves Immunoregulatory Effect of Polysaccharide from Seeds of Plantago asiatica L.
In-situ administration of dendritic cells following argon–helium cryosurgery enhances specific antiglioma immunity in mice
Surface-Layer Protein-Enhanced Immunotherapy Based on Cell Membrane-Coated Nanoparticles for the Effective Inhibition of Tumor Growth and Metastasis
Structure–Activity and Structure–Property Relationship and Exploratory in Vivo Evaluation of the Nanomolar Keap1–Nrf2 Protein–Protein Interaction Inhibitor
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Customer Reviews
Have you used Mouse IL-12(p70) ELISA Kit PicoKine®?
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1 Reviews For Mouse IL-12(p70) ELISA Kit PicoKine®
0
Good Mouse IL-12 ELISA Kit--Daisy Dai University of Miami Surgery Lab Director / Manager
Excellent
Source: Biocompare.com
| Application | ELISA |
|---|---|
| Starting Material | Crispr MEK and STAT3 KO CAFs |
| Tips | This kit doesn't provide PC |
"We investigate how alterations in RAS and STAT3 signaling pathways affect the tumor stroma and immune microenvironment in pancreatic cancer. This kit was used to detect the IL-12 level in CM from Crispr MEK and STAT3 KO CAFs. The whole process was about 7 hours including sample preparation."
Daisy Dai
Verified customer
Submitted 2020-07-30
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Mouse IL-12(p70) ELISA Kit PicoKine®
Question
Q: how to thaw whole blood sample for IL12A ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-09-11
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL12A for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-09-11
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-05-21
Question
Q: Can IL12A ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-06-25
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-06-25
Question
Q: can you suggest the dilution ratio of serum samples for detection of IL12A in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2017-04-03
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL12A ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-04-03
