Mouse IL-2/Interleukin-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

IL-2 ELISA antibody pairs, Mouse, for 5 plates

Mouse IL-2/Interleukin-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Il2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1 pg/ml.

Product Info Summary

SKU: EZ0398
Size: For 5 plates, 96 wells each, reagents only, plates are not included
Reactive Species: Mouse
Application: ELISA

Product Name

Mouse IL-2/Interleukin-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all IL-2 products

SKU/Catalog Number

EZ0398

Size

For 5 plates, 96 wells each, reagents only, plates are not included

*Question: How many samples can I assay/run in this kit?

Description

Mouse IL-2/Interleukin-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Il2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse IL-2/Interleukin-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0398)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A21-Q169

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0398 is reactive to Il2 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Application Guarantee

EZ0398 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ0398-CA Mouse anti- mouse Il2 monoclonal antibody (Capture Antibody) 500 µl, 4 µg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ0398-DA Biotinylated goat anti- mouse Il2 polyclonal antibody (Detection Antibody) 500 µl, 0.075 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ0398-ST Lyophilized recombinant mouse Il2 standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0330.1020.2000.3990.6341.3571.7262.042

Data Example Images

Gene/Protein Information For Il2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P04351

Gene ID

16183

Gene Name

Il2

Full Name

Interleukin-2

Weight

19.4kDa

Superfamily

IL-2 family

Alternative Names

Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF Il2|IL, Il-2|interleukin 2|interleukin-2|T-cell growth factor|TCGF

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il2, check out the Il2 Infographic

Il2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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Boster offers all the reagents you need for your immunostaining and western blotting experiments. We've got everything from buffers to lysates to kits, including our popular and well-cited BCA, CCK-8, and MTT assay kits.

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