Mouse IL-2 ELISA Kit PicoKine™

Mouse IL-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml. Cited in 35 publication(s).

Product Info Summary

SKU: EK0398
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Mouse IL-2 ELISA Kit PicoKine™

See all IL-2 products

SKU/Catalog Number

EK0398

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IL-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse IL-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0398)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A21-Q169

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0398 is reactive to Il2 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0398 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0398-CAP Anti-Mouse Il2 Pre-coated 96-well strip microplate 1
EK0398-ST Mouse Il2 Standard 2 vials, 10 ng/tube
EK0398-DA Mouse Il2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0330.1020.2000.3990.6341.3571.7262.042

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-2, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of IL-2 in samples: 24373408 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IL-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3715646740166452
Standard deviation2.18.5837.7%52.610.6237.06
CV (%)5.7%5.5%7.7%6.5%6.4%8.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 137403639381.584.1%
Sample 21561621661551594.492.8%
Sample 34674484704634628.451.8%
*number of samples for each test n=16.

Gene/Protein Information For Il2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P04351

Gene ID

16183

Gene Name

Il2

Full Name

Interleukin-2

Weight

19.4kDa

Superfamily

IL-2 family

Alternative Names

Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF Il2|IL, Il-2|interleukin 2|interleukin-2|T-cell growth factor|TCGF

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il2, check out the Il2 Infographic

Il2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0398 has been cited in 35 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Qiu-yue Fang,Shu-ping Chen,Jun-qiao Wang,Xiao-jun Huang,Qi-xing Nie,Glyn O. Philips,Steve W. Cui,Ya-jing Li,Shao-ping Nie,Fractions from natural Cordyceps sinensis alleviated intestinal injury in Cyclophosphamide-induced mice,Bioactive Carbohydrates and Dietary Fibre,2021,100271,ISSN 2212-6198,https://doi.org/10.1016/j.bcdf.2021.100271.
Species: Mouse
EK0398 usage in article: APP:ELISA, SAMPLE:SMALL INTESTINE, DILUTION:NA

Xi Y,Ma Y,Xie B,Di A,Xu S,Luo X,Wang C,Dai H,Yan G,Qi Z.Vitamin D3 combined with antibody agents suppresses alloreactive memory T-cell responses to induce heart allograft long-term survival.Transpl Immunol.2021 Feb 13:101374.doi:10.1016/j.trim.2021.101374
Species: Mouse
EK0398 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Zhang Z,Song X,Zhang Z,Li H,Duan Y,Zhang H,Lu H,Luo C, Wang M.The molecular characterization and immune protection of adhesion protein 65 (AP65) of Trichomonas vaginalis.Microb Pathog.2021 Jan 20:104750.doi:10.1016/j.micpath.2021.104750.Epub ahead of prin
Species: Mouse,Rat
EK0398 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Mingyue Shen,Xianxiang Chen,Lixin Huang,Qiang Yu,Yi Chen,Jianhua Xie,Sulfated Mesona chinensis Benth polysaccharide enhance the immunomodulatory activities of cyclophosphamide-treated mice,Journal of Functional Foods,Volume 76,2021,104321,ISSN 1756-4646,h
Species: Mouse
EK0398 usage in article: APP:ELISA, SAMPLE:SPLENIC LYMPHOCYTES, DILUTION:NA

Dai,J,Chen,J,Qi,J, et al. Konjac Glucomannan from Amorphophallus konjac enhances immunocompetence of the cyclophosphamide‐induced immunosuppressed mice. Food Sci Nutr. 2020; 00: 1– 8. https://doi.org/ 10.1002/fsn3.2038
Species: Mouse
EK0398 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Sun H, Fei L, Zhu B, Shi M. Quick and improved immune responses to inactivated H9N2 avian influenza vaccine by purified active fraction of Albizia julibrissin saponins. BMC Vet Res. 2020 Nov 7;16(1):427. doi: 10.1186/s12917-020-02648-1. PMID: 33160337; PM
Species: Mouse,Chicken
EK0398 usage in article: APP:ELISA, SAMPLE:SPLENOCYTES, DILUTION:NA

Huang L,Shen M,Wu T,Yu Y,Yu Q,Chen Y,Xie J.Mesona chinensis Benth polysaccharides protect against oxidative stress and immunosuppression in cyclophosphamide-treated mice via MAPKs signal transduction pathways.Int J Biol Macromol.2020 Jun 1;152:766-774. do
Species: Mesona chinensis, Mouse
EK0398 usage in article: APP:ELISA, SAMPLE:SPLEEN, DILUTION:NA

Feng-Yang Chen, Li-Fei Zhou, Xiao-Yu Li, Shi-Fang Xu, Li-Juan Gao, Hong-Xiang Sun and Yi-Ping Ye

il-8 regulates the stemness properties of cancer stem cells in the small-cell lung cancer cell line h446

Immune Protection of Rhoptry Protein 21 (ROP21) of Toxoplasma gondii as a DNA Vaccine Against Toxoplasmosis

Role of serotonin on the intestinal mucosal immune response to stress-induced diarrhea in weaning mice

Oroxylin A inhibits the generation of Tregs in non-small cell lung cancer

Genetically Engineered Virus Nanofibers as an Efficient Vaccine for Preventing Fungal Infection

Immunological adjuvant effect of the peptide fraction from the larvae of Musca domestica

Hybrid phage displaying SLAQVKYTSASSI induces protection against?Candida albicans?challenge in BALB/c mice

Yang T, Shi W, Wumaierniyazi Z, Shan L, Miao W, Wu G, Zhou B, Yusup A, Upur H, Aikemu A. Oncotarget. 2017 Oct 6;8(54):92682-92698. doi: 10.18632/oncotarget.21563. eCollection 2017 Nov 3. Enhanced antitumor efficacy and reduced toxicity of Abnormal...

Liang W, Mao S, Sun S, Li M, Li Z, Yu R, Ma T, Gu J, Zhang J, Taniguchi N, Li W. Front Immunol. 2018 Jan 29;9:78. doi: 10.3389/fimmu.2018.00078. eCollection 2018. Core Fucosylation of the T Cell Receptor Is Required for T Cell Activation

ang J, Liu W, Chen Z, Chen H. Biomed Pharmacother. 2017 Mar 26;90:160-170. doi: 10.1016/j.biopha.2017.03.059. Physicochemical characterization of the oolong tea polysaccharides with high molecular weight and their synergistic effects in combinatio...

Xie Ft, Cao Js, Zhao J, Yu Y, Qi F, Dai Xc. Transpl Immunol. 2015 Oct;33(2):69-77. Doi: 10.1016/J.Trim.2015.05.003. Epub 2015 May 20. Ido Expressing Dendritic Cells Suppress Allograft Rejection Of Small Bowel Transplantation In Mice By Expansion O...

Chen F, Ni Y, Ye Y, Sun H, Li X, Xu S. Eur J Pharmacol. 2012 Jun 15;685(1-3):186-97. Doi: 10.1016/J.Ejphar.2012.04.012. Epub 2012 Apr 20. Stephanthraniline A Inhibits The Proliferation And Activation Of T Cells In Vitro And In Vivo.

He Rr, Wang M, Wang Cz, Chen Bt, Lu Cn, Yao Xs, Chen Jx, Kurihara H. J Agric Food Chem. 2011 Apr 27;59(8):3730-7. Doi: 10.1021/Jf104982Y. Epub 2011 Mar 14. Protective Effect Of Apple Polyphenols Against Stress-Provoked Influenza Viral Infection In...

Cheng K, Wu Z, Gao B, Xu J. Cell Biochem Biophys. 2014 Nov;70(2):1305-9. Doi: 10.1007/S12013-014-0055-9. Analysis Of Influence Of Baicalin Joint Resveratrol Retention Enema On The Tnf-??, Siga, Il-2, Ifn-?? Of Rats With Respiratory Syncytial Virus...

Liang Y, Liu Hh, Chen Yj, Sun H. J Med Food. 2014 Apr;17(4):439-46. Doi: 10.1089/Jmf.2013.2846. Epub 2014 Mar 4. Antitumor Activity Of The Protein And Small Molecule Component Fractions From Agrocybe Aegerita Through Enhancement Of Cytokine Produc...

Hu W,, Jing P, Wang L, Zhang Y, Yong J, Wang Y. Bmc Complement Altern Med. 2015 Apr 15;15:119. Doi: 10.1186/S12906-015-0642-3. The Positive Effects Of Ginsenoside Rg1 Upon The Hematopoietic Microenvironment In A D-Galactose-Induced Aged Rat Model.

Chen F, Ni Y, Ye Y, Sun H, Li X, Xu S. Int Immunopharmacol. 2010 Oct;10(10):1153-60. Doi: 10.1016/J.Intimp.2010.06.021. Epub 2010 Jul 4. Comparison Of Immunosuppressive Activity Of Stephanoside E And Its Aglycone From Stephanotis Mucronata In Vitro.

Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (????????????

Zhu Hc, Qiu T, Liu Xh, Dong Wc, Weng Xd, Hu Ch, Kuang Yl, Gao Rh, Dan C, Tao T. Cell Immunol. 2012;274(1-2):12-8. Doi: 10.1016/J.Cellimm.2012.02.012. Epub 2012 Mar 14. Tolerogenic Dendritic Cells Generated By Relb Silencing Using Shrna Prevent Acu...

Guida F, Luongo L, Aviello G, Palazzo E, De Chiaro M, Gatta L, Boccella S, Marabese I, Zjawiony Jk, Capasso R, Izzo Aa, De Novellis V, Maione S. Mol Pain. 2012 Aug 23;8:60. Doi: 10.1186/1744-8069-8-60. Salvinorin A Reduces Mechanical Allodynia And...

Liu Sh, Shen Xh, Wei Xf, Mao Xh, Huang T. Immunopharmacol Immunotoxicol. 2011 Mar;33(1):100-6. Doi: 10.3109/08923973.2010.483520. Epub 2010 May 20. Immunomodulatory Activity Of Butanol Extract From Solanum Lyratum In Tumor-Bearing Mice.

Eker Ss, Yavasci Eo, Cangur S, Kirli S, Sarandol E. Acta Neuropsychiatr. 2014 Oct;26(5):291-7. Doi: 10.1017/Neu.2014.13. Can Bdnf And Il-2 Be Indicators For The Diagnosis In Schizophrenic Patients With Depressive Symptoms?

Zhong G, Cheng X, Long H, He L, Qi W, Xiang T, Zhao Z, Zhu B. J Transl Med. 2013 Mar 21;11:71. Doi: 10.1186/1479-5876-11-71. Dynamically Expressed Microrna-15B Modulates The Activities Of Cd8+ T Lymphocytes In Mice With Lewis Lung Carcinoma.

Sofian M, Aghakhani A, Farazi Aa, Banifazl M, Eslamifar A, Rashidi N, Khadem Sadegh A, Ramezani A. Hepat Mon. 2012 Dec;12(12):E6156. Doi: 10.5812/Hepatmon.6156. Epub 2012 Dec 29. Serum Profile Of T Helper 1 And T Helper 2 Cytokines In Hepatitis C ...

Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induce...

Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.

Meng Fy, Ning Yl, Qi J, He Z, Jie J, Lin Jj, Huang Yj, Li Fs, Li Xh. Int J Mol Sci. 2014 Mar 24;15(3):5140-62. Doi: 10.3390/Ijms15035140. Structure And Antitumor And Immunomodulatory Activities Of A Water-Soluble Polysaccharide From Dimocarpus Lon...

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7 Customer Q&As for Mouse IL-2 ELISA Kit PicoKine™

Question

Q: Can IL-2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

L. Lewis

Verified customer

Asked: 2019-08-12

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-08-12

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of IL-2 in Mouse plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2019-01-09

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-01-09

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

M. Walker

Verified customer

Asked: 2018-08-22

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-08-22

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

T. Green

Verified customer

Asked: 2018-04-24

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-04-24

Question

Q: how do I analyze ELISA data? I measured IL-2 level in plasma.

Verified Customer

Verified customer

Asked: 2016-05-23

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2016-05-23

Question

Q: if the enzyme conjugated IL-2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-2 antigen?

Verified Customer

Verified customer

Asked: 2016-05-22

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-05-22

Question

Q: What is the optimal O.D. value for IL-2 ELISA kit? I performed your IL-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL-2 even though the O.D. values are not very high?

P. Wright

Verified customer

Asked: 2014-01-07

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-2 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of IL-2 that can be declared/interpreted as positive by the above standard is 1pg/ml.

Boster Scientific Support

Answered: 2014-01-07

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