Overview
| Product Name |
Mouse IL-21 ELISA Kit PicoKine™
|
| Catalog# |
EK0797 |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Mouse IL-21 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il21 in cell culture supernatants and serum.. Sensitivity: 10pg/ml. |
| Cite This Product |
Mouse IL-21 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0797)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Mouse |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants and serum. |
| Sensitivity |
<10 pg/ml |
| Assay Range |
62.5 pg/ml - 4,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: E.coli; Immunogen sequence: P25-S146 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For Il21 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q5SUE2 |
|---|
| Gene Name | Il21 |
|---|
| Molecular Weight | 16811 MW |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Il21, check out the Il21 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Il21: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Il21 infographic
Kit Components And Assay QC Data Details Of Mouse IL-21 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0797-CAP |
Anti-Mouse Il21 Pre-coated 96-well strip microplate | 1 |
| EK0797-ST | Mouse Il21 Standard | 2 vials, 10 ng/tube |
| EK0797-DA | Mouse Il21 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0797 Mouse IL-21 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.086 | 0.176 | 0.257 | 0.382 | 0.644 | 1.103 | 1.778 | 2.343 |
We measured random samples of Mouse IL-21 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 176 | 862 | 1322 | 188 | 946 | 1262 |
| Standard deviation | 9.32 | 43.96 | 96.50 | 9.96 | 52.97 | 107.27 |
| CV (%) | 5.3% | 8.5% | 7.3% | 5.3% | 5.6% | 8.5% |
We measured the performance consistency of Mouse IL-21 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 176 | 199 | 176 | 180 | 182 | 9.52 | 5.2% |
| Sample 2 | 862 | 737 | 773 | 850 | 805 | 52.25 | 6.4% |
| Sample 3 | 1322 | 1296 | 1226 | 1149 | 1248 | 67.2 | 5.3% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 3
| Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration |
PubMed ID 27411313 |
| Shen, Z., Yang, H., Yang, S., Wang, W., Cui, X., Zhou, X.,..., & Liu, J. (2017). Hepatitis B virus persistence in mice reveals IL-21 and IL-33 as regulators of viral clearance. Nature Communications, 8(1), 2119. doi: 10.1038/s41467-017-02304-7 |
PubMed ID 29242561 |
| Yu Ht, Tian D, Wang Jy, Guo Cx, Li Y, Wang X, Li D, Zhang Fm, Zhuang M, Ling H. Plos One. 2014 Dec 29;9(12):E115047. Doi: 10.1371/Journal.Pone.0115047. Ecollection 2014. An Hiv-1 Envelope Immunogen With W427S Mutation In Cd4 Binding Site Induced M... |
PubMed ID 25546013 |
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Questions and answers from customers related to EK0797 Mouse IL-21 ELISA Kit PicoKine™
5 Related Questions
Question
What would be the starting dilution of this antibody EK0797?
Verified Customer
Asked: 2020-04-09
Answer
We would recommend for the product EK0797 kit a 2 fold dilution for plasma samples
Boster Scientific Support
Answered: 2020-04-09
Question
Q: if the enzyme conjugated IL-21 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-21 antigen?
Verified Customer
Asked: 2020-04-06
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-04-06
Question
Q: how to thaw whole blood sample for IL-21 ELISA after freezing?
Verified Customer
Asked: 2020-03-17
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-21 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-03-17
Question
Q: how do I analyze ELISA data? I measured IL-21 level in serum.
L. Adams
Asked: 2018-10-02
Boster Scientific Support
Answered: 2018-10-02
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
C. Mitchell
Asked: 2018-03-11
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-03-11
