Mouse IL-21/Interleukin-21 ELISA Kit PicoKine®

IL-21 ELISA kit for Mouse

Mouse IL-21 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il21 in cell culture supernatants and serum. Sensitivity: 10pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0797
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants and serum.

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Product Name

Mouse IL-21/Interleukin-21 ELISA Kit PicoKine®

View all IL-21 ELISA kits

SKU/Catalog Number

EK0797

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IL-21 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il21 in cell culture supernatants and serum. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse IL-21/Interleukin-21 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0797)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: P25-S146

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0797 is reactive to Il21 in Mouse samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK0797 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL-21

Interleukin-21 is a protein that in humans is encoded by the IL21 gene. Interleukin 21 is a cytokine that has potent regulatory effects on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. By radiation hybrid analysis, the IL21 gene is mapped to chromosome 4q26-q27, near the IL2 gene. IL21 stimulates B-cell proliferation in an anti-CD40-dependent manner but inhibits proliferation stimulated by IL4 plus anti-IgM. The biologic effects of IL21 on NK cells differ between species, with human IL21 enhancing NK cell proliferation and murine IL21 inhibiting NK cell proliferation. IL21 is a cytokine highly expressed by mouse T (H)17 cells.

Kit Components

Catalog Number Description Quantity
EK0797-CAP Anti-Mouse Il21 Pre-coated 96-well strip microplate 1
EK0797-ST Mouse Il21 Standard 2 vials, 10 ng/tube
EK0797-DA Mouse Il21 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0560.0950.1760.2570.3820.6441.1031.878

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IL-21/Interleukin-21 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)17686213221889461262
Standard deviation9.3243.9696.509.9652.97107.27
CV (%)5.3%8.5%7.3%5.3%5.6%8.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-21 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11761991761801829.525.2%
Sample 286273777385080552.256.4%
Sample 31322129612261149124867.25.3%
*number of samples for each test n=16.

Gene/Protein Information For Il21 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9ES17

Gene ID

60505

Gene Name

Il21

Full Name

Interleukin-21

Weight

16.811kDa

Superfamily

IL-15/IL-21 family

Alternative Names

CVID11; IL21; IL-21; IL-21Za11interleukin-21; interleukin 21; interleukin-21 isoform; Za11 Il21|IL-21|interleukin 21|interleukin-21

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il21, check out the Il21 Infographic

Il21 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il21: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0797 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Characterization of IL-21-expressing recombinant hepatitis B virus (HBV) as a therapeutic agent targeting persisting HBV infection

IL-21-based therapies induce clearance of hepatitis B virus persistence in mouse models

Upregulation of microRNA-219-5p relieves ulcerative colitis through balancing the differentiation of Treg/Th17 cells

A polysaccharide from natural Cordyceps sinensis regulates the intestinal immunity and gut microbiota in mice with cyclophosphamide-induced intestinal injury

Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration

Lactobacillus plantarum NCU116 Attenuates Cyclophosphamide-Induced Immunosuppression and Regulates Th17/Treg Cell Immune Responses in Mice

Ganoderma atrum polysaccharide ameliorates intestinal mucosal dysfunction associated with autophagy in immunosuppressed mice

Cultured Cordyceps sinensis polysaccharides modulate intestinal mucosal immunity and gut microbiota in cyclophosphamide-treated mice

The effectiveness of cyclooxygenase-2 inhibitors and evaluation of angiogenesis in the model of experimental colorectal cancer

Prior transient exposure to interleukin-21 delivered by recombinant adeno-associated virus vector protects mice from hepatitis B virus persistence

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7 Customer Q&As for Mouse IL-21/Interleukin-21 ELISA Kit PicoKine®

Question

Does EK0797 contain native human biological materials?

Verified customer

Asked: 2021-11-10

Answer

The Mouse IL-21/Interleukin-21 ELISA Kit PicoKine® (EK0797) doesn't contain native human biological materials.

Boster Scientific Support

Answered: 2021-11-11

Question

Does EK0797 contain native human biological materials?

Verified customer

Asked: 2021-11-10

Answer

The Mouse IL-21/Interleukin-21 ELISicA Kit PoKine® (EK0797) doesn't contain native human biological materials.

Boster Scientific Support

Answered: 2021-11-11

Question

What would be the starting dilution of this antibody EK0797?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

We would recommend for the product EK0797 kit a 2 fold dilution for plasma samples

Boster Scientific Support

Answered: 2020-04-09

Question

Q: if the enzyme conjugated IL-21 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-21 antigen?

Verified Customer

Verified customer

Asked: 2020-04-06

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-04-06

Question

Q: how to thaw whole blood sample for IL-21 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-21 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-03-17

Question

Q: how do I analyze ELISA data? I measured IL-21 level in serum.

L. Adams

Verified customer

Asked: 2018-10-02

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-10-02

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

C. Mitchell

Verified customer

Asked: 2018-03-11

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-03-11

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