Click image to see more details
Product Info Summary
| SKU: | EK0797 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Mouse IL-21/Interleukin-21 ELISA Kit PicoKine®
SKU/Catalog Number
EK0797
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-21 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il21 in cell culture supernatants and serum. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IL-21/Interleukin-21 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0797)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: P25-S146
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0797 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0797 is reactive to IL21 in Mouse samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0797 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-21
Interleukin-21 is a protein that in humans is encoded by the IL21 gene. Interleukin 21 is a cytokine that has potent regulatory effects on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. By radiation hybrid analysis, the IL21 gene is mapped to chromosome 4q26-q27, near the IL2 gene. IL21 stimulates B-cell proliferation in an anti-CD40-dependent manner but inhibits proliferation stimulated by IL4 plus anti-IgM. The biologic effects of IL21 on NK cells differ between species, with human IL21 enhancing NK cell proliferation and murine IL21 inhibiting NK cell proliferation. IL21 is a cytokine highly expressed by mouse T (H)17 cells.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0797-CAP | Anti-Mouse IL21 Pre-coated 96-well strip microplate | 1 |
| EK0797-ST | Mouse IL21 Standard | 2 vials, 10 ng/tube |
| EK0797-DA | Mouse IL21 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.056 | 0.095 | 0.176 | 0.257 | 0.382 | 0.644 | 1.103 | 1.878 |
Data Example Images
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Mouse IL-21 PicoKine ELISA Kit standard curve A
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-21, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 62.5 pg/ml..
Some articles we found to cite concentrations of IL-21 in samples: 25419350 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-21/Interleukin-21 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 176 | 862 | 1322 | 188 | 946 | 1262 |
| Standard deviation | 9.32 | 43.96 | 96.50 | 9.96 | 52.97 | 107.27 |
| CV (%) | 5.3% | 8.5% | 7.3% | 5.3% | 5.6% | 8.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-21 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 176 | 199 | 176 | 180 | 182 | 9.52 | 5.2% |
| Sample 2 | 862 | 737 | 773 | 850 | 805 | 52.25 | 6.4% |
| Sample 3 | 1322 | 1296 | 1226 | 1149 | 1248 | 67.2 | 5.3% |
Protein Target Info & Infographic
Gene/Protein Information For IL21 (Source: Uniprot.Org, NCBI)
Gene Name
IL21
Full Name
Interleukin-21
Weight
18.653kDa
Superfamily
IL-15/IL-21 family
Alternative Names
CVID11; IL21; IL-21; IL-21Za11interleukin-21; interleukin 21; interleukin-21 isoform; Za11 IL21 CVID11, IL-21, Za11 interleukin 21 interleukin-21
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL21, check out the IL21 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL21: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse IL-21/Interleukin-21 ELISA Kit PicoKine® (EK0797)
Hello CJ!
EK0797 has been cited in 15 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Characterization of IL-21-expressing recombinant hepatitis B virus (HBV) as a therapeutic agent targeting persisting HBV infection
IL-21-based therapies induce clearance of hepatitis B virus persistence in mouse models
Upregulation of microRNA-219-5p relieves ulcerative colitis through balancing the differentiation of Treg/Th17 cells
A polysaccharide from natural Cordyceps sinensis regulates the intestinal immunity and gut microbiota in mice with cyclophosphamide-induced intestinal injury
Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration
Lactobacillus plantarum NCU116 Attenuates Cyclophosphamide-Induced Immunosuppression and Regulates Th17/Treg Cell Immune Responses in Mice
Ganoderma atrum polysaccharide ameliorates intestinal mucosal dysfunction associated with autophagy in immunosuppressed mice
Cultured Cordyceps sinensis polysaccharides modulate intestinal mucosal immunity and gut microbiota in cyclophosphamide-treated mice
The effectiveness of cyclooxygenase-2 inhibitors and evaluation of angiogenesis in the model of experimental colorectal cancer
Prior transient exposure to interleukin-21 delivered by recombinant adeno-associated virus vector protects mice from hepatitis B virus persistence
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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Submit your question
7 Customer Q&As for Mouse IL-21/Interleukin-21 ELISA Kit PicoKine®
Question
Does EK0797 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Mouse IL-21/Interleukin-21 ELISA Kit PicoKine® (EK0797) doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Does EK0797 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Mouse IL-21/Interleukin-21 ELISicA Kit PoKine® (EK0797) doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
What would be the starting dilution of this antibody EK0797?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
We would recommend for the product EK0797 kit a 2 fold dilution for plasma samples
Boster Scientific Support
Answered: 2020-04-09
Question
Q: if the enzyme conjugated IL-21 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-21 antigen?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-04-06
Question
Q: how to thaw whole blood sample for IL-21 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-21 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-03-17
Question
Q: how do I analyze ELISA data? I measured IL-21 level in serum.
L. Adams
Verified customer
Asked: 2018-10-02
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-10-02
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
C. Mitchell
Verified customer
Asked: 2018-03-11
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-03-11
