This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Il21 antibody (A00459-1) performs well in ELISA, IHC and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
IHC analysis of IL-21/Il21 using anti-IL-21/Il21 antibody (A00459-1).
IL-21/Il21 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IL-21/Il21 Antibody (A00459-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
IL-21/Il21 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IL-21/Il21 Antibody (A00459-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Bundle Save
Anti-IL-21/Il21 Antibody
Il21 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, IHC |
Tested Applications
Il21 antibody was positively detected by IHC in:
mouse spleen tissue, rat spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse, Rat
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
IHC
IF
ELISA
Mouse
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Product Information
Product Name
Anti-IL-21/Il21 Antibody
View all IL21 Antibodies
View all IL21 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse IL-21/Il21 recombinant protein (Position: H18-S146).
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Calculated Molecular Weight
16.8 kDa
Background of Il21
Interleukin 21 (IL-21) is a protein that in humans is encoded by the IL21 gene. This gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. Dysregulation of this gene plays a role in multiple immune-mediated diseases including lupus, psoriasis and chronic inflammatory diseases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Cite This Product
Anti-IL-21/Il21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00459-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
88%
Moderate Homology
Cow
67%
Low homology
Sheep
67%
Low homology
Dog
66%
Low homology
Human
63%
Low homology
Monkey
62%
Low homology
Rabbit
56%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for Il21 antibody
Review
Have you used Anti-IL-21/Il21 Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-IL-21/Il21 Antibody performs excellently in relevant applications for ELISA, IHC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00459-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026