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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Mouse Leptin Receptor ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse Leptin Receptor ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Lepr in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Leptin Receptor ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0440)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: L22-G839
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0440 is reactive to Lepr in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0440 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Leptin R
Leptin receptor (or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family. Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene (LEPR) might contribute to obesity or obesity-related diseases. Leptin is also involved in the regulation of blood pressure through the leptin receptor.
|EK0440-CAP||Anti-Mouse Lepr Pre-coated 96-well strip microplate||1|
|EK0440-ST||Mouse Lepr Standard||2 vials, 10 ng/tube|
|EK0440-DA||Mouse Lepr Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse Leptin receptor PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Leptin Receptor ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Leptin R in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Lepr (Source: Uniprot.Org, NCBI)
type I cytokine receptor family
B219; CD295 antigen; CD295; DB; DKFZp686B1731; huB219; LEPR; LEP-R; Leptin R; leptin receptor; LeptinR; OB R; OB receptor; OB-R; OBRCD295 Lepr|LE, LEPROTb, Modb, Modb1, O, OB-, OB-RGRP, Obr, db, diabetes, obese-, obese-like, obl, Lepr|leptin receptor|leptin receptor|B219|LEP-R|OB receptor|leptin receptor gene-related protein*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Lepr, check out the Lepr Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Lepr: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0440 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
In utero exposure to di-(2-ethylhexyl) phthalate induces metabolic disorder and increases fat accumulation in visceral depots of C57BL/6J mice offspring
Exogenous leptin affects sperm parameters and impairs blood testis barrier integrity in adult male mice
The effect of intermittent hypoxia on bodyweight, serum glucose and cholesterol in obesity mice
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