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Product Info Summary
| SKU: | EK0470 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Mouse NGF/NGF Beta ELISA Kit PicoKine®
SKU/Catalog Number
EK0470
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse NGF/NGF Beta ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ngf in cell culture supernatants and serum. Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse NGF/NGF Beta ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0470)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: S122-G241
Sensitivity
<1 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0470 for more details
Cross-reactivity
There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4.
Reactive Species
EK0470 is reactive to NGF in Mouse samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0470 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of beta-NGF
Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0470-CAP | Anti-Mouse NGF Pre-coated 96-well strip microplate | 1 |
| EK0470-ST | Mouse NGF Standard | 2 vials, 10 ng/tube |
| EK0470-DA | Mouse NGF Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.033 | 0.096 | 0.138 | 0.238 | 0.443 | 0.740 | 1.239 | 1.852 |
Data Example Images
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Mouse NGF/NGF beta PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect beta-NGF, Dilution ratio of 1:1, concentration in serum is less than the lowest standard, 31.2 pg/ml..
Some articles we found to cite concentrations of beta-NGF in samples: 25296021 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse NGF/NGF Beta ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 99 | 235 | 910 | 99 | 244 | 967 |
| Standard deviation | 6.43 | 17.39 | 70.07 | 6.43 | 18.05 | 80.26 |
| CV (%) | 6.5% | 7.4% | 7.7% | 6.5% | 7.4% | 8.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of beta-NGF in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 99 | 87 | 83 | 82 | 87 | 6.75 | 7.7% |
| Sample 2 | 235 | 231 | 230 | 203 | 224 | 12.69 | 5.6% |
| Sample 3 | 910 | 887 | 898 | 936 | 907 | 18.22 | 2% |
Protein Target Info & Infographic
Gene/Protein Information For NGF (Source: Uniprot.Org, NCBI)
Gene Name
NGF
Full Name
Beta-nerve growth factor
Weight
26.959kDa
Superfamily
NGF-beta family
Alternative Names
Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428 NGF Beta-NGF, HSAN5B, NGF nerve growth factor beta-nerve growth factor|nerve growth factor (beta polypeptide)|nerve growth factor, beta subunit|pro-nerve growth factor long
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NGF, check out the NGF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NGF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse NGF/NGF Beta ELISA Kit PicoKine® (EK0470)
Hello CJ!
EK0470 has been cited in 16 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Low‐level laser therapy 810‐nm up‐regulates macrophage secretion of neurotrophic factors via PKA‐CREB and promotes neuronal axon regeneration in vitro
Effect of Zhuang Jing Decoction on Learning and Memory Ability in Aging Rats
Magnetic NGF-Releasing PLLA/Iron Oxide Nanoparticles Direct Extending Neurites and Preferentially Guide Neurites along Aligned Electrospun Microfibers
Investigation of glycosaminoglycan mimetic scaffolds for neurite growth
AVP(4-8) Improves Cognitive Behaviors and Hippocampal Synaptic Plasticity in the APP/PS1 Mouse Model of Alzheimer’s Disease
PET Detection of Cerebral Necrosis Using an Infarct-Avid Agent 2-Deoxy-2-[ 18 F]Fluoro- d -Glucaric Acid (FGA) in a Mouse Model of the Brain Stroke
Nanofibrous gelatine scaffolds integrated with nerve growth factor‐loaded alginate microspheres for brain tissue engineering
The effect of neutralization of nerve growth factor (NGF) on bladder and urethral dysfunction in mice with spinal cord injury
Post‐injury bladder management strategy influences lower urinary tract dysfunction in the mouse model of spinal cord injury
Magnetic NGF-releasing PLLA/iron oxide nanoparticles direct extending neurites and preferentially guide neurites along aligned electrospun microfibers.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
16 Customer Q&As for Mouse NGF/NGF Beta ELISA Kit PicoKine®
Question
Hi I have a question for EK0470. My sample type is mouse plasma and prepared in a following way: A plasma sample will be prepared as follows: whole blood will be placed into an (+4°C) precooled K2-EDTA tube (and kept cold). The tube will be gently turned a few times to fully mix the blood and centrifuged at 2000 g (+4°C) for 10 min within 30 minutes. Questions: 1) Is the plasma isolation protocol ok for this kit? 2) The kit states that plasma should be stored at -20. Can we store the plasma also at -80C? 3) would you have any experience what dilution would be used for the mouse plasma samples (are 1:2, 1:5 and 1:10 possibly good dilutions to be tested)? Thank you for the help, and looking forward to hearing from you.
Verified customer
Asked: 2022-07-18
Answer
Thank you for your inquiry. Please see the following information. 1) Is the plasma isolation protocol ok for this kit? The protocol is OK. 2) The kit states that plasma should be stored at -20. Can we store the plasma also at -80C? The plasma can be stored at -80C. 3) would you have any experience what dilution would be used for the mouse plasma samples (are 1:2, 1:5 and 1:10 possibly good dilutions to be tested)? 1:1 is suggested (e.g. Add 50μl sample to 50 μl sample diluent buffer). We hope our response has satisfied your request. Should there be any questions, please feel free to contact us. We would be more than happy to help.
Boster Scientific Support
Answered: 2022-07-18
Question
Is the plasma isolation protocol okay for EK0470?
Verified customer
Asked: 2022-07-14
Answer
Yes, the plasma isolation protocol is okay for the Mouse NGF/NGF Beta ELISA Kit PicoKine® (EK0470).
Boster Scientific Support
Answered: 2022-07-18
Question
EK0470 states that plasma should be stored at -20, can we store the plasma also at -80C?
Verified customer
Asked: 2022-07-14
Answer
For the Mouse NGF/NGF Beta ELISA Kit PicoKine® (EK0470), the plasma can be stored at -80C.
Boster Scientific Support
Answered: 2022-07-18
Question
Would you have any experience what dilution would be used for mouse plasma samples (are 1:2, 1:5 and 1:10 possibly good dilutions to be tested) in EK0470?
Verified customer
Asked: 2022-07-14
Answer
For the Mouse NGF/NGF Beta ELISA Kit PicoKine® (EK0470), 1:1 is suggested (e.g. Add 50μl sample to 50 μl sample diluent buffer).
Boster Scientific Support
Answered: 2022-07-18
Question
Q: how to thaw whole blood sample for beta-NGF ELISA after freezing?
M. Singh
Verified customer
Asked: 2020-12-09
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test beta-NGF for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-12-09
Question
Q: if the enzyme conjugated beta-NGF antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the beta-NGF antigen?
Verified Customer
Verified customer
Asked: 2020-07-27
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-07-27
Question
Q: What is the optimal O.D. value for beta-NGF ELISA kit? I used your beta-NGF ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain beta-NGF even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-06-23
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this beta-NGF ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of beta-NGF that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2020-06-23
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
T. Taylor
Verified customer
Asked: 2020-05-22
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-05-22
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis beta-NGF using the EK0470 Mouse beta-NGF Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-04-17
Question
Q: Can beta-NGF ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-04-16
Question
Are you selling the coated microplates of EK0470 kit separately if damage is sustained during shipment?
Verified customer
Asked: 2019-08-08
Answer
Yes, we are able to sell the coated microplates of the Mouse NGF/NGF Beta ELISA Kit PicoKine EK0470 separately. You may purchase using the sku EK0470-CAP and also include the lot number on the Purchase Order.
Boster Scientific Support
Answered: 2019-08-09
Question
Was thimerosal used in any of the reagents for the EK0470 kit?
Verified customer
Asked: 2019-07-30
Answer
The Mouse NGF/NGF Beta ELISA Kit PicoKine EK0470 does not contain thimerosal in any of its reagents.
Boster Scientific Support
Answered: 2019-07-30
Question
Was thimerosal used in any of the reagents for the EK0470 kit?
Verified customer
Asked: 2019-07-30
Answer
The Mouse NGF/NGF Beta ELISA Kit PicoKine EK0470 does not contain thimerosal in any of its reagents.
Boster Scientific Support
Answered: 2019-07-30
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
T. Baker
Verified customer
Asked: 2018-06-16
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-06-16
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-02-17
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-02-17
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
L. Martin
Verified customer
Asked: 2017-12-01
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2017-12-01
