Mouse PECAM-1/CD31/PECAM1 PicoKine® Quick ELISA Kit

Product Name

Mouse PECAM-1/CD31/PECAM1 PicoKine® Quick ELISA Kit

See all CD31/PECAM-1 products

SKU/Catalog Number

FEK0874

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse PECAM-1/CD31/PECAM1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse PECAM-1/CD31/PECAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Cite This Product

Mouse PECAM-1/CD31/PECAM1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0874)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: E18-K590

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK0874 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0874 is reactive to Pecam1 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Application Guarantee

FEK0874 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CD31/PECAM-1

PECAM-1 is an important target GP in DITP.1 Platelet/endothelial cell adhesion molecule-1(PECAM-1) is a 130-kD member of the Ig gene superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and selective T-cell subsets.2 Platelet endothelial cell adhesion molecule-1(PECAM-1/CD31) is a member of the immunoglobulin(Ig) superfamily that has distinctive features of an immunoreceptor based upon its genomic structure and the presence of intrinsic immunoreceptor tyrosine inhibitory motifs(ITIMs) in its ligand binding polypeptide.3 PECAM-1 has recently been shown to contain functional immunoreceptor tyrosine-based inhibitory motifs(ITIMs) within its cytoplasmic domain, and co-ligation of PECAM-1 with the T-cell antigen receptor(TCR) results in tyrosine phosphorylation of PECAM-1, recruitment of Src homology 2 domain-containing protein tyrosine phosphatase-2(SHP-2), and attenuation of TCR-mediated cellular signaling.4 The standard used in this kit is the product of gene recombinant expression(extracellular part), consisting of 563 amino acids(E28-K590) with the molecular weight of 65.5KDa. After glycosylating, its molecular weight changes to 91-100KDa.

Kit Components

Catalog number	Description	Quantity	Volume
FEK0874-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
EK0874-ST	Mouse CD31/PECAM-1 Standard	2	10 ng/tube
FEK0874-A	Mouse Pecam1 Antibody A	1	3ml
FEK0874-B	Mouse Pecam1 Antibody B	1	3ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
O.D.	0.043	0.136	0.192	0.312	0.545	0.908	1.441	2.033

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse PECAM-1/CD31/PECAM1 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CD31/PECAM-1 in ELISA kits from four different production batches/lots.

Gene/Protein Information For Pecam1 (Source: Uniprot.Org, NCBI)

Alternative Names

adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule PECAM1 CD31, CD31/EndoCAM, GPIIA, PECA1, PECAM-1, endoCAM platelet and endothelial cell adhesion molecule 1 platelet endothelial cell adhesion molecule|CD31 |platelet endothelial cell adhesion molecule-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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