Mouse Periostin/OSF2 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Periostin/OSF2. 96wells/kit, with removable strips. Cited in 2 publication(s).
Mouse Periostin/OSF2 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Mouse Periostin/OSF2 ELISA Kit PicoKine™
See all Periostin/OSF-2 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Mouse Periostin/POSTN with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Periostin/POSTN tissue specificity: Preferentially expressed in periosteum and periodontal ligament. Also expressed in the developing and adult heart. .|
|Cite This Product||Mouse Periostin/OSF2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1187)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: N24-Q836|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For Postn (Source: Uniprot.org, NCBI)
|NCBI Gene Id||50706|
|Species Of This Entry||Mouse|
|Alternative Names||Periostin/OSF-2|Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Acetylation; Sulphation; Carboxylation; Reduction; Glycosylation; Gamma-carboxylation; Methylation|
|Gene Location||3|3 C, on Chromasome 3, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Postn (Source: Uniprot.org, NCBI)
|Protein Function||Induces cell attachment and spreading and plays a role in cell adhesion (PubMed:10404027). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (PubMed:20181949).|
|Tissue Specificity||Preferentially expressed in periosteum and periodontal ligament (PubMed:10404027). Also expressed in the developing and adult heart (PubMed:11335131).|
|Related Diseases||Malignant Neoplasms; Neoplasms; Tissue Adhesions; Neoplasm Metastasis; Carcinoma; Cell Invasion; Fibrosis; Neoplasm Invasiveness; Periosteal Disorder; Inflammation; Tumor Angiogenesis; Asthma; Lung Neoplasms; Mammary Neoplasms; Malignant Neoplasm Of Breast; Malignant Paraganglionic Neoplasm; Hypertrophy; Heart Failure; Malignant Squamous Cell Neoplasm; Myocardial Infarction|
|Related Pathways||Cell Adhesion; Pathogenesis; Angiogenesis; Regeneration; Cell Proliferation; Localization; Cell Migration; Secretion; Osteoblast Differentiation; Cell Growth; Cell Cycle; Ossification; Heart Development; Bone Development; Tissue Regeneration; Tissue Development; Tooth Eruption; Cell Differentiation; Aging; Reverse Transcription|
|Background||Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/ beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.|
|Scientific References||PMID: 8363580 by Takeshita S., et al. Osteoblast-specific factor 2: cloning of a putative bone adhesion protein with homology with the insect protein fasciclin I.|
PMID: 10404027 by Horiuchi K., et al. Identification and characterization of a novel protein, periostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta.
Kit Components And Assay QC Data Details Of Mouse Periostin/OSF2 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK1187-CAP||96-well plate precoated with anti-Mouse Periostin/OSF-2 antibody||1|
|EK1187-ST||lyophilized recombinant Mouse Periostin/OSF-2 standard||10ng/tube|
|EK1187-DA||biotinylated anti-Mouse Periostin/OSF-2 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1187 Mouse Periostin/OSF2 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse Periostin/OSF2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse Periostin/OSF2 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 2
|Krencik R, Hokanson Kc, Narayan Ar, Dvornik J, Rooney Ge, Rauen Ka, Weiss La, Rowitch Dh, Ullian Em. Sci Transl Med. 2015 May 6;7(286):286Ra66. Doi: 10.1126/Scitranslmed.Aaa5645. Dysregulation Of Astrocyte Extracellular Signaling In Costello Syndr...||PubMed ID 25947161|
|Li Y, Xie K, Chen H, Wang G, Yu Y. J Surg Res. 2015 Jun 1;196(1):136-48. Doi: 10.1016/J.Jss.2015.02.042. Epub 2015 Feb 27. Hydrogen Gas Inhibits High-Mobility Group Box 1 Release In Septic Mice By Upregulation Of Heme Oxygenase 1.||PubMed ID 25818978|
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