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Product Info Summary
| SKU: | FEK0727 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Mouse CXCL4/PF4 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0727
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CXCL4/PF4 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse CXCL4/PF4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Mouse CXCL4/PF4 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0727)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: V30-S105
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0727 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0727 is reactive to Pf4 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK0727 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CXCL4/PF4
Platelet factor 4(PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine(C-X-C motif) ligand 4(CXCL4). The PF4 gene was localized on map to 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0727-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0727-ST | Mouse CXCL4/PF4 Standard | 2 | 10 ng/tube |
| FEK0727-A | Mouse Pf4 Antibody A | 1 | 3ml |
| FEK0727-B | Mouse Pf4 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.050 | 0.148 | 0.224 | 0.379 | 0.685 | 1.249 | 1.544 | 2.098 |
Data Example Images
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Mouse PF4 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CXCL4/PF4 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 34 | 241 | 440 | 30 | 261 | 456 |
| Standard deviation | 1.73 | 11.57 | 28.16 | 1.92 | 16.18 | 31.46 |
| CV (%) | 5.1% | 4.8% | 6.4% | 6.4% | 6.2% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CXCL4/PF4 in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 34 | 33 | 32 | 34 | 33 | 0.83 | 2.5% |
| Sample 2 | 241 | 265 | 259 | 244 | 252 | 9.08 | 3.6% |
| Sample 3 | 440 | 440 | 458 | 462 | 450 | 18.45 | 4.1% |
Protein Target Info & Infographic
Gene/Protein Information For Pf4 (Source: Uniprot.Org, NCBI)
Gene Name
Pf4
Full Name
Platelet factor 4
Weight
10.845kDa
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; CXCL4; CXCL4iroplact; Iroplact; MGC138298; Oncostatin-A; PF4; PF-4; platelet factor 4; SCYB4oncostatin-A PF4 CXCL4, PF-4, SCYB4 platelet factor 4 platelet factor 4|C-X-C motif chemokine 4|chemokine (C-X-C motif) ligand 4|iroplact|oncostatin-A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Pf4, check out the Pf4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Pf4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CXCL4/PF4 PicoKine® Quick ELISA Kit (FEK0727)
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