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Product Info Summary
| SKU: | EK1835 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Mouse REG3A ELISA Kit PicoKine®
SKU/Catalog Number
EK1835
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse REG3A ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Reg3a in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse REG3A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1835)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: E27-Q175
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1835 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1835 is reactive to REG3A in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK1835 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of REG3A
Regenerating islet-derived protein 3 alpha is a protein that in humans is encoded by the REG3A gene. It is mapped to 6 C3; 6 34.76 cM. This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. Multiple alternatively spliced transcript variants encoding the same protein have been described for this gene but the full length nature of some transcripts is not yet known.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1835-CAP | Anti-Mouse REG3A Pre-coated 96-well strip microplate | 1 |
| EK1835-ST | Mouse REG3A Standard | 2 vials, 10 ng/tube |
| EK1835-DA | Mouse REG3A Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.020 | 0.065 | 0.132 | 0.258 | 0.432 | 0.753 | 1.095 | 1.807 |
Data Example Images
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Mouse REG3A PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect REG3A, Dilution ratio of 1:10, concentration in serum and plasma is around 1600 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse REG3A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 30 | 174 | 502 | 32 | 209 | 528 |
| Standard deviation | 2.19 | 9.05 | 23.59 | 2.05 | 11.70 | 23.23 |
| CV (%) | 7.3% | 5.2% | 4.7% | 6.4% | 5.6% | 4.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of REG3A in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 30 | 35 | 32 | 36 | 33 | 2.38 | 7.2% |
| Sample 2 | 174 | 156 | 153 | 148 | 157 | 9.80 | 6.2% |
| Sample 3 | 502 | 518 | 455 | 436 | 477 | 33.42 | 7.0% |
Protein Target Info & Infographic
Gene/Protein Information For REG3A (Source: Uniprot.Org, NCBI)
Gene Name
REG3A
Full Name
Regenerating islet-derived protein 3-alpha
Weight
19.395kDa
Alternative Names
hepatocarcinoma-intestine-pancreas; HIPpancreatitis-associated protein; Human proislet peptide; Lithostathine 3; pancreatic beta cell growth factor; Pancreatitis-associated protein 1; PAP homologous protein; PAP1FLJ18565; PAP2; PAPPAP-H; PBCGF; proliferation-inducing protein 34; proliferation-inducing protein 42; Reg III-alpha; REG3; Reg3A; REG-3-alpha; regenerating islet-derived 3 alpha; regenerating islet-derived protein 3-alpha; Regenerating islet-derived protein III-alpha; REG-III REG3A HIP, HIP/PAP, INGAP, PAP, PAP-H, PAP1, PBCGF, REG-III, REG3 regenerating family member 3 alpha regenerating islet-derived protein 3-alpha|PAP homologous protein|REG-3-alpha|hepatocarcinoma-intestine-pancreas|hepatointestinal pancreatic protein|human proislet peptide|pancreatic beta cell growth factor|pancreatitis-associated protein 1|proliferation-inducing protein 34|proliferation-inducing protein 42|reg III-alpha|regenerating islet-derived 3 alpha|regenerating islet-derived protein III-alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on REG3A, check out the REG3A Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for REG3A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse REG3A ELISA Kit PicoKine® (EK1835)
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No publications found for EK1835
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Mouse REG3A ELISA Kit PicoKine®
Question
Q: Can REG3A ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
B. Mitchell
Verified customer
Asked: 2018-11-22
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-11-22
Question
Q: What is the optimal O.D. value for REG3A ELISA kit? I performed your REG3A ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain REG3A even though the O.D. values are not very high?
W. Clark
Verified customer
Asked: 2018-10-01
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this REG3A ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of REG3A that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-10-01
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
P. Lee
Verified customer
Asked: 2018-04-29
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-04-29
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
M. Baker
Verified customer
Asked: 2016-07-05
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-07-05
