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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA) and urine.|
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Mouse SAA/SAA1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse SAA/SAA1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Saa1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 150pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse SAA/SAA1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1190)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: G20-Y122
1.56 ng/ml - 100 ng/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1190 is reactive to Saa1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
EK1190 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Saa1
Serum amyloid A protein (SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.
|EK1190-CAP||Anti-Mouse Saa1 Pre-coated 96-well strip microplate||1|
|EK1190-ST||Mouse Saa1 Standard||2 vials, 100 ng/tube|
|EK1190-DA||Mouse Saa1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse SAA PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Saa1, Dilution ratio of 1:5, concentration in serum is 90-125ng/ml.
Some articles we found to cite concentrations of Saa1 in samples: 24520213 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse SAA/SAA1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Saa1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Saa1 (Source: Uniprot.Org, NCBI)
Serum amyloid A-1 protein
Serum Amyloid A1 Saa1|Sa, Saa-1, Saa2|serum amyloid A 1|serum amyloid A-1 protein|serum amyloid A 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Saa1, check out the Saa1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Saa1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK1190 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse
Fluid supplementation accelerates epithelial repair during chemical colitis
Therapeutic effects of rosuvastatin in hypercholesterolemic prediabetic mice in the absence of low density lipoprotein receptor
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