Mouse SAA/SAA1 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse SAA. 96wells/kit, with removable strips.
Mouse SAA/SAA1 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum, plasma(heparin, EDTA) and urine.|
|Sample Volume:||100μl per well|
|Product Name||Mouse SAA/SAA1 ELISA Kit PicoKine™
See all Saa1 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Mouse Serum Amyloid A1/SAA1 with <150pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA) and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Serum Amyloid A1/SAA1 tissue specificity: Detected in blood plasma (at protein level). Detected in liver. .|
|Cite This Product||Mouse SAA/SAA1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1190)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum, plasma(heparin, EDTA) and urine.|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: G20-Y122|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For Saa1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||20208|
|Species Of This Entry||Mouse|
|Protein Name||Serum amyloid A-1 protein|
|Alternative Names||Saa1|Serum Amyloid A1/A2|
|Post Tranlational Modifications||Cleavage; Nitration; Phosphorylation; Methylation; Glycosylation|
|Gene Location||7 B3|7 30.54 cM, on Chromasome 7, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Saa1 (Source: Uniprot.org, NCBI)
|Protein Function||Major acute phase protein.|
|Tissue Specificity||Detected in blood plasma (at protein level). Detected in liver.|
|Related Diseases||Amyloidosis; Inflammation; Arthritis; Rheumatoid Arthritis; Secondary Amyloidosis; Acute-phase Reaction; Familial Mediterranean Fever; Brucellosis; Malignant Neoplasms; Liver Carcinoma; Plaque, Amyloid; Neoplasms; Kidney Diseases; Amyloid Deposition; Carcinoma; Amyloid Nephropathy; Liver Neoplasms; Inflammatory Response; Cardiovascular Diseases|
|Related Pathways||Pathogenesis; Inflammatory Response; Acute-phase Response; Immune Response; Translation; Localization; Secretion; Amyloid Fibril Formation; Transport; Proteolysis; Cell Adhesion; Tissue Remodeling; Cholesterol Efflux; Lipid Binding; Cell Cycle; Mrna Stabilization; Methylation; Cytokine Production; Cell Activation; T Cell Activation|
|Background||Serum amyloid A protein(SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.|
|Scientific References||PMID: 3013853 by Lowell C.A., et al. Structure of the murine serum amyloid A gene family. Gene conversion.|
PMID: 3857624 by Yamamoto K., et al. Complete primary structures of two major murine serum amyloid A proteins deduced from cDNA sequences.
Kit Components And Assay QC Data Details Of Mouse SAA/SAA1 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK1190-CAP||96-well plate precoated with anti-Mouse Saa1 antibody||1|
|EK1190-ST||lyophilized recombinant Mouse Saa1 standard||100ng/tube|
|EK1190-DA||biotinylated anti-Mouse Saa1 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1190 Mouse SAA/SAA1 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse SAA/SAA1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse SAA/SAA1 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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