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Mouse SCF ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse SCF. 96wells/kit, with removable strips. Cited in 1 publication(s).
Mouse SCF ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0509 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Mouse |
| Sample Type: | cell culture supernates, serum and plasma(EDTA). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <10pg/ml |
| Assay Range: | 31.2pg/ml-2000pg/ml |
Introduction
Chromogranin A (a protein of 439 amino acid which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors.
Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
Overview
| Product Name | Mouse SCF ELISA Kit PicoKine™
See all Kitlg primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0509 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Mouse Scf/KITLG with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Scf/KITLG tissue specificity: |
| Cite This Product | Mouse SCF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0509) |
Product Specs
| Reactivity/Species | Mouse |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, serum and plasma(EDTA). |
| Sensitivity | <10pg/ml |
| Assay Range | 31.2pg/ml-2000pg/ml |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: K26-A190 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P20826 |
|---|---|
| Gene Name | Kitlg |
| Protein Name | Kit ligand |
| Subcellular Localization | Isoform 1: Cell membrane ; Single-pass type I membrane protein . |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Mouse SCF ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0509-CAP | 96-well plate precoated with anti-Mouse Kitlg antibody | 1 |
| EK0509-ST | lyophilized recombinant Mouse Kitlg standard | 10ng/tube |
| EK0509-DA | biotinylated anti-Mouse Kitlg antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0509 Mouse SCF ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 2000 | 2000 | 2000 | 2000 | 2000 | 2000 | 2000 |
| O.D. | 0.055 | 0.098 | 0.124 | 0.215 | 0.399 | 0.768 | 1.468 | 2.236 |
We measured random samples of Mouse SCF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 75 | 190 | 820 | 81 | 186 | 861 |
| Standard deviation | 7%5 | 10.26 | 42.64 | 6.96 | 12.46 | 45.4%3 |
| CV(%) | 7% | 5.4% | 5.2% | 8.6% | 6.7% | 5.3% |
We measured the performance consistency of Mouse SCF ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 75 | 88 | 85 | 89 | 84 | 5.53 | 6.5% |
| Sample 2 | 190 | 214 | 186 | 211 | 200 | 12.37 | 6.1% |
| Sample 3 | 820 | 795 | 798 | 947 | 840 | 62.52 | 7.4% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 1
| GM-CSF Inhibits c-Kit and SCF Expression by Bone Marrow-Derived Dendritic Cells | PubMed ID 28261209 |
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Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
Contact Information
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| (888) 466-3604 |
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