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Product Info Summary
| SKU: | EK1635 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
Product info
Product Name
Mouse SCGB1A1/uteroglobin ELISA Kit PicoKine®
View all Uteroglobin/SCGB1A1 ELISA kits
SKU/Catalog Number
EK1635
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse SCGB1A1/uteroglobin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Scgb1a1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse SCGB1A1/uteroglobin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1635)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: D22-F96
Sensitivity
<1 pg/ml
Assay Range
4.7 pg/ml - 300 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1635 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1635 is reactive to SCGB1A1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Application Guarantee
EK1635 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Uteroglobin/SCGB1A1
SCGB1A1 (secretoglobin family 1A member 1) also known as Uteroglobin is a protein that in humans is encoded by the SCGB1A1 gene. SCGB1A1 is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is specifically expressed in Clara cells in the lungs. Numerous studies demonstrated that UG is a multifunctional protein with antiinflammatory/ immunomodulatory properties. It inhibits soluble phospholipase A (2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, UG manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1635-CAP | Anti-Mouse SCGB1A1 Pre-coated 96-well strip microplate | 1 |
| EK1635-ST | Mouse SCGB1A1 Standard | 2 vials, 10 ng/tube |
| EK1635-DA | Mouse SCGB1A1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 4.7 | 9.4 | 18.8 | 37.5 | 75 | 150 | 300 |
| O.D. | 0.031 | 0.084 | 0.167 | 0.257 | 0.421 | 0.730 | 1.239 | 1.920 |
Data Example Images
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Mouse SCGB1A1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Uteroglobin/SCGB1A1, Dilution ratio of 1:200, concentration in serum and plasma is around 20 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse SCGB1A1/uteroglobin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 14 | 51 | 122 | 13 | 47 | 124 |
| Standard deviation | 0.64 | 2.49 | 7.32 | 0.79 | 2.35 | 8.3 |
| CV (%) | 4.6% | 4.9% | 6% | 6.1% | 5% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Uteroglobin/SCGB1A1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 14 | 13 | 16 | 14 | 14 | 1.08 | 7.7% |
| Sample 2 | 51 | 57 | 51 | 56 | 53 | 2.77 | 5.2% |
| Sample 3 | 122 | 120 | 106 | 115 | 115 | 6.17 | 5.3% |
Protein Target Info & Infographic
Gene/Protein Information For SCGB1A1 (Source: Uniprot.Org, NCBI)
Gene Name
SCGB1A1
Full Name
Uteroglobin
Weight
9.994kDa
Superfamily
secretoglobin family
Alternative Names
Blastokinin; CC10; CC10Urine protein 1; CC16; CCPBP; CCSP; CCSPCCPBP; Clara cell phospholipid-binding protein; Clara cells 10 kDa secretory protein; Clara; PCB-BP; SCGB1A1; Secretoglobin family 1A member 1; secretoglobin, family 1A, member 1 (uteroglobin); UGB; UGBUP-1; UP1; Urinary protein 1; Uteroglobin SCGB1A1 CC10, CC16, CCPBP, CCSP, UGB, UP-1, UP1 secretoglobin family 1A member 1 uteroglobin|blastokinin|clara cell phospholipid-binding protein|clara cells 10 kDa secretory protein|secretoglobin, family 1A, member 1 (uteroglobin)|urinary protein 1|urine protein 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SCGB1A1, check out the SCGB1A1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SCGB1A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse SCGB1A1/uteroglobin ELISA Kit PicoKine® (EK1635)
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No publications found for EK1635
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Mouse SCGB1A1/uteroglobin ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-08-05
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-08-05
Question
Q: Can Uteroglobin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
V. Robinson
Verified customer
Asked: 2020-07-21
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-07-21
Question
Q: What is the optimal O.D. value for Uteroglobin ELISA kit? I used your Uteroglobin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Uteroglobin even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-05-29
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Uteroglobin ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of Uteroglobin that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2020-05-29
Question
Q: how do I analyze ELISA data? I have performed Uteroglobin level in serum.
Verified Customer
Verified customer
Asked: 2020-03-15
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-03-15
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
S. Hernandez
Verified customer
Asked: 2019-11-18
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-11-18
Question
Q: is it okay to use citrate plasma as samples in Mouse Uteroglobin Picokine® ELISA Kit (Catalog # EK1635)?
A. Kaur
Verified customer
Asked: 2019-07-21
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Uteroglobin causing disruption of its protein structure. Uteroglobin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Uteroglobin ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-07-21
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Uteroglobin in Mouse serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
P. Zhang
Verified customer
Asked: 2018-10-04
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Uteroglobin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-10-04
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
T. Bhatt
Verified customer
Asked: 2015-06-22
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2015-06-22
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Uteroglobin using the EK1635 Mouse Uteroglobin Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2014-03-28
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2014-03-28
