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Product Info Summary
| SKU: | A02201-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | IHC, WB, ELISA (Cap) |
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Product info
Product Name
Anti-Uteroglobin/Scgb1a1 Antibody Picoband®
SKU/Catalog Number
A02201-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Uteroglobin/Scgb1a1 Antibody Picoband® catalog # A02201-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Uteroglobin/Scgb1a1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02201-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat Uteroglobin recombinant protein (Position: S20-V96).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02201-2 is reactive to Scgb1a1 in Rat
Observed Molecular Weight
10 kDa
Calculated molecular weight
10.4 kDa
Background of Scgb1a1
Uteroglobin, also known as secretoglobin family 1A member 1 (SCGB1A1), is a protein that in humans is encoded by the SCGB1A1 gene. This gene encodes a member of the secretoglobin family of small secreted proteins. The encoded protein has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02201-2 is guaranteed for IHC, WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA(Cap), 1-5μg/ml
Positive Control
WB: rat lung tissue, rat lung tissue
IHC: rat lung tissue
Validation Images & Assay Conditions
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Western blot analysis of Uteroglobin using anti-Uteroglobin antibody (A02201-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysate,
Lane 2: rat lung tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Uteroglobin antigen affinity purified polyclonal antibody (Catalog # A02201-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Uteroglobin at approximately 10KD. The expected band size for Uteroglobin is at 10KD.
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IHC analysis of Uteroglobin using anti-Uteroglobin antibody (A02201-2). Uteroglobin was detected in paraffin-embedded section of rat lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Uteroglobin Antibody (A02201-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Sandwich ELISA - Recombinant rat SCGB1A1 protein standard curve.
Use in combination with reagents from Rat SCGB1A1 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1636).
Specific Publications For Anti-Uteroglobin/Scgb1a1 Antibody Picoband® (A02201-2)
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1 Customer Q&As for Anti-Uteroglobin/Scgb1a1 Antibody Picoband®
Question
We are currently using anti-Uteroglobin/Scgb1a1 antibody A02201-2 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
The anti-Uteroglobin/Scgb1a1 antibody (A02201-2) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-02
