Mouse SLPI ELISA Kit PicoKine™

Mouse SLPI ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse SLPI in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1996
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin)

Product Name

Mouse SLPI ELISA Kit PicoKine™

See all Slpi products

SKU/Catalog Number

EK1996

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse SLPI ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse SLPI in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse SLPI ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1996)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: G26-M131

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1996 is reactive to SLPI in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin)

Application Guarantee

EK1996 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1996-CAP Anti-Mouse SLPI Pre-coated 96-well strip microplate 1
EK1996-ST Mouse SLPI Standard 2 vials, 2 ng/tube
EK1996-DA Mouse SLPI Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0180.1270.1970.4140.7691.3302.0692.463

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Slpi, Dilution ratio of 1:4000, concentration in serum is 1.6-6.4ug/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse SLPI ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5921493862237958
Standard deviation2.4811.3453.473.8413.7560.35
CV (%)4.2%5.3%5.7%6.2%5.8%6.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Slpi in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 159566566614.156.8%
Sample 221421519422321110.685.0%
Sample 3938982104095897938.243.9%
*number of samples for each test n=16.

Gene/Protein Information For SLPI (Source: Uniprot.Org, NCBI)

Uniprot ID

P97430

Gene ID

20568

Gene Name

SLPI

Full Name

Antileukoproteinase

Weight

14.308kDa

Alternative Names

ALK1; ALPProtease inhibitor WAP4; antileukoproteinase; BLPISeminal proteinase inhibitor; HUSI; HUSI-I; Mucus proteinase inhibitor; secretory leukocyte peptidase inhibitor; secretory leukocyte protease inhibitor (antileukoproteinase); Secretory leukocyte protease inhibitor; SLPI; WAP four-disulfide core domain protein 4; WAP4HUSI-1; WFDC4MPI Slpi|ALP|secretory leukocyte peptidase inhibitor|antileukoproteinase|secretory leukocyte protease inhibitor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SLPI, check out the SLPI Infographic

SLPI infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SLPI: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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8 Customer Q&As for Mouse SLPI ELISA Kit PicoKine™

Question

Q: how to analyze ELISA data? I measured SLPI level in plasma.

Verified Customer

Verified customer

Asked: 2020-10-13

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-10-13

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

C. Mehta

Verified customer

Asked: 2020-03-13

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-03-13

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-12-22

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-12-22

Question

Q: if the enzyme conjugated SLPI antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the SLPI antigen?

M. Dhawan

Verified customer

Asked: 2019-10-04

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-10-04

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-08-08

Question

Q: can I use citrate plasma as samples in Mouse SLPI Picokine® ELISA Kit (Catalog # EK1996)?

Verified Customer

Verified customer

Asked: 2018-09-08

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of SLPI causing degradation of its protein structure. SLPI may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the SLPI ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-09-08

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of SLPI in Mouse plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2017-03-14

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the SLPI ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2017-03-14

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

T. Johnson

Verified customer

Asked: 2014-11-16

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2014-11-16

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