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Product Info Summary
| SKU: | FEK0772 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
| Assay Time: | 90 Minutes |
How Quick Picokine(R) ELISA kits work: Get data in 90 minutes
Product info
Product Name
Mouse SPINT1/HAI-1 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0772
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse SPINT1/HAI-1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Spint1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4～C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Mouse SPINT1/HAI-1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0772)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E30-L507
Sensitivity
<12 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0772 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0772 is reactive to Spint1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
FEK0772 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Spint1
Kunitz-type protease inhibitor 1 is an enzyme that in humans is encoded by the SPINT1 gene. It is a member of the Kunitz family of serine protease inhibitors. The SPINT1 gene encodes an inhibitor of hepatocyte growth factor activator (HGFAC) and matriptase and is involved in degradation of the extracellular matrix. SPINT1 is produced as an active membrane-associated protein and is proteolytically cleaved and secreted. Alternative splicing results in multiple variants encoding different isoforms.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0772-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| FEK0772-ST | Mouse Spint1 Standard | 2 | 10 ng/tube |
| FEK0772-A | Mouse Spint1 Antibody A | 1 | 3ml |
| FEK0772-B | Mouse Spint1 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.021 | 0.092 | 0.155 | 0.298 | 0.569 | 1.026 | 1.843 | 3.069 |
Data Example Images
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Mouse SPINT1/HAI-1 PicoKine Quick ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse SPINT1/HAI-1 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 199 | 707 | 1860 | 185 | 728 | 1735 |
| Standard deviation | 14.72 | 41 | 119.04 | 16.4%1 | 48.1%3 | 140.53 |
| CV (%) | 7.4% | 5.8% | 6.4% | 8.6% | 6.2% | 8.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Spint1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 199 | 180 | 196 | 188 | 190 | 7.39 | 3.8% |
| Sample 2 | 707 | 699 | 666 | 710 | 695 | 17.5 | 2.5% |
| Sample 3 | 1860 | 1696 | 2012 | 1755 | 1830 | 120 | 6.5% |
Protein Target Info & Infographic
Gene/Protein Information For Spint1 (Source: Uniprot.Org, NCBI)
Gene Name
Spint1
Full Name
Weight
56.6 kDa
For more info on Spint1, check out the Spint1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Spint1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us support@bosterbio.com.
Specific Publications For Mouse SPINT1/HAI-1 PicoKine® Quick ELISA Kit (FEK0772)
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