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Product Info Summary
|Size:||For 5 plates, 96 wells each|
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Mouse TNFRSF9/4-1BB ELISA Kit EZ-Set™ (DIY Antibody Pairs)
For 5 plates, 96 wells each
*Question: How many samples can I assay/run in this kit?
Mouse TNFRSF9/4-1BB ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Tnfrsf9 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse TNFRSF9/4-1BB ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ1143)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: V24-L187
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EZ1143 is reactive to Tnfrsf9 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EZ1143 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EZ1143-CA||Rabbit anti- mouse Tnfrsf9 polyclonal antibody (Capture Antibody)||500 µl, 0.4 mg/mL (recommended dilution 1:100)||0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol|
|EZ1143-DA||Biotinylated goat anti- mouse Tnfrsf9 polyclonal antibody (Detection Antibody)||500 µl, 5.5 µg/mL (recommended dilution 1:100)||0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol|
|EZ1143-ST||Lyophilized recombinant mouse Tnfrsf9 standard||10 ng/tube×2|
|AR1103||Avidin-Biotin-Peroxidase Complex (ABC)||500 µl (recommended dilution 1:100)|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. 96 well microplate (Cat# AR1100)
- 6. Plate Sealers.
- 7. Capture Antibody Diluent: PBS.
- 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 11. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
- *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse TNFRSF9/4-1BB EZ-Set ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
Gene/Protein Information For Tnfrsf9 (Source: Uniprot.Org, NCBI)
Tumor necrosis factor receptor superfamily member 9
4-1BB ligand receptor; 41BB; 4-1BB; CD137 antigen; CD137; CD137MGC2172; CDw137; FLJ43501; ILA; ILAhomolog of mouse 4-1BB; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNFRSF9; tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9 Tnfrsf9|4-1BB, A930040I11Rik, AA408498, AI325004, CDw137, Cd137, ILA, Ly6, Ly63|tumor necrosis factor receptor superfamily, member 9|tumor necrosis factor receptor superfamily member 9|4-1BB ligand receptor|CD137 antigen|T-cell antigen 4-1BB|secreted CD137 antigen*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Tnfrsf9, check out the Tnfrsf9 Infographic
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In this infographic you will see the following information for Tnfrsf9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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