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Product Info Summary
| SKU: | EK0541 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
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Product info
Product Name
Mouse VEGF ELISA Kit PicoKine™
SKU/Catalog Number
EK0541
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse VEGF ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Vegfa in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse VEGF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0541)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A27-R190
Sensitivity
<2 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0541 is reactive to Vegfa in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0541 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0541-CAP | Anti-Mouse Vegfa Pre-coated 96-well strip microplate | 1 |
| EK0541-ST | Mouse Vegfa Standard | 2 vials, 10 ng/tube |
| EK0541-DA | Mouse Vegfa Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.035 | 0.074 | 0.131 | 0.211 | 0.353 | 0.660 | 1.201 | 1.936 |
Data Example Images
Click image to see more details
Mouse VEGF PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse VEGF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 40 | 179 | 538 | 40 | 171 | 574 |
| Standard deviation | 1.92 | 9.48 | 36.58 | 2.6 | 9.57 | 48.79 |
| CV (%) | 4.8% | 5.3% | 6.8% | 6.5% | 5.6% | 8.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Vegfa in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 40 | 34 | 35 | 37 | 36 | 2.29 | 6.3% |
| Sample 2 | 179 | 204 | 186 | 197 | 191 | 9.65 | 5% |
| Sample 3 | 538 | 463 | 494 | 471 | 491 | 29.15 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For Vegfa (Source: Uniprot.Org, NCBI)
Uniprot ID
Q00731
Gene ID
22339
Gene Name
Vegfa
Full Name
Vascular endothelial growth factor A
Weight
25.283kDa
Superfamily
PDGF/VEGF growth factor family
Alternative Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor Vegfa|V, VEGF12, VEGF16, VEGF18, Veg, Vegf, Vpf|vascular endothelial growth factor A|vascular endothelial growth factor A|vascular permeability factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Vegfa, check out the Vegfa Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Vegfa: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Mouse VEGF ELISA Kit PicoKine™ (EK0541)
EK0541 has been cited in 50 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The enhancement of cancer stem cell properties of MCF-7 cells in 3D collagen scaffolds for modeling of cancer and anti-cancer drugs
Species: Mouse
Vascular contributions to 16p11.2 deletion autism syndrome modeled in mice
Species: Mouse
Fu DN,Wang Y,Yu LJ, Liu MJ,Zhen D.Silenced long non-coding RNA activated by DNA damage elevates microRNA-495-3p to suppress atherosclerotic plaque formation via reducing Krüppel-like factor 5.Exp Cell Res.2021 Feb 23: 112519.doi:10.1016/j.yexcr.2021.11251
Species: Mouse
EK0541 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Liu Y,Feng M,Cai J,Li S,Dai X,Shan G,Wu S.Repurposing bortezomib for choroidal neovascularization treatment via antagonizing VEGF-A and PDGF-D mediated signaling.Exp Eye Res.2021 Jan 18;204:108446.doi:10.1016/j.exer.2021.108446.Epub ahead of print.PMID:33
Species: Mouse
EK0541 usage in article: APP:ELISA, SAMPLE:RPE TISSUE, DILUTION:NA
Serine racemase deficiency attenuates choroidal neovascularization and reduces nitric oxide and VEGF levels by retinal pigment epithelial cells
Intravenous injection of l-aspartic acid β-hydroxamate attenuates choroidal neovascularization via anti-VEGF and anti-inflammation
Two tandem repeats of mHSP70407-426 enhance therapeutic antitumor effects of a recombined vascular endothelial growth factor (VEGF) protein vaccine
Down-regulation of HPV18 E6, E7, or VEGF expression attenuates malignant biological behavior of human cervical cancer cells
Inhibition of tumor angiogenesis by interferon-γ by suppression of tumor-associated macrophage differentiation
Autocrine VEGF signalling on M2 macrophages regulates PD-L1 expression for immunomodulation of T cells
Endostar, a modified recombinant human endostatin, exhibits synergistic effects with dexamethasone on angiogenesis and hepatoma growth
A novel dressing seeded with embryonic artery CD133+ cells and loaded with the Sirt1 agonist SRT1720 accelerates the healing of diabetic ischemic ulcers
Modulation of Salmonella Tumor-Colonization and Intratumoral Anti-angiogenesis by Triptolide and Its Mechanism
Induced migration of endothelial cells into 3D scaffolds by chemoattractants secreted by pro-inflammatory macrophages in situ
Efficiency of calcium phosphate composite nanoparticles in targeting Ehrlich carcinoma cells transplanted in mice
Relationship between single nucleotide polymorphisms in the 3?-untranslated region of the vascular endothelial growth factor gene and susceptibility to diabetic peripheral neuropathy in China
Inhibition of lung tumor growth in nude mice by siRNACD31?targeting PECAM-1
Microvesicles secreted from human multiple myeloma cells promote angiogenesis
Zou J, Wang N, Liu M, Bai Y, Wang H, Liu K, Zhang H, Xiao X, Wang K. J Cell Mol Med. 2018 May;22(5):2692-2705. doi: 10.1111/jcmm.13552. Epub 2018 Mar 7. Nucleolin mediated pro
Sun Y,, Chen X, Zhang X, Shen X, Wang M, Wang X,, Liu WC, Liu CF, Liu J, Liu W, Jin X,. Front Mol Neurosci. 2017 Aug 14;10:257. doi: 10.3389/fnmol.2017.00257. eCollection 2017. β2-Adrenergic Receptor-Mediated HIF-1α Upregulation Mediates Blood Br...
Li M, Wang S, Wang S, Zhang L, Wu D, Yang R, Ji A, Li Y, Wang J. Exp Ther Med. 2017 Aug;14(2):1732-1738. doi: 10.3892/etm.2017.4651. Epub 2017 Jun 22. Occludin downregulation in high glucose is regulated by SSTR2 via the VEGF/NRP1/Akt signaling pa...
Li Z, Ding Q, Ling LP, Wu Y, Meng DX, Li X, Zhang CQ. World J Gastroenterol. 2018 Feb 21;24(7):819-832. doi: 10.3748/wjg.v24.i7.819. Metformin attenuates motility, contraction, and fibrogenic response of hepatic stellate cells in vivo and in vitro...
Wen D, Huang X, Zhang M, Zhang L, Chen J, Gu Y, Hao Cm. Plos One. 2013 Dec 3;8(12):E82336. Doi: 10.1371/Journal.Pone.0082336. Ecollection 2013. Resveratrol Attenuates Diabetic Nephropathy Via Modulating Angiogenesis.
Ge Y, Ding Y, Zhang J, Li Z, Li Z. Int J Clin Exp Med. 2014 Oct 15;7(10):3578-82. Ecollection 2014. Effect Of Angiogenesis Inhibitor Su6668 In Combination With 5-Fu On Liver Metastasis From Transplantation Tumors Of Human Colorectal Cancer In Nude...
Lu G, Zhang G, Zhang C, Chen C, Liu R. Radiat Oncol. 2013 Mar 26;8:74. Doi: 10.1186/1748-717X-8-74. A Study Of 131Iodine-Labeling Of Histamine-Indomethacin: Its In Vivo Therapeutic Effect And Anti-Tumor Mechanisms In Lewis-Bearing Lung Cancer.
Yang W, Zhang Y, Yu J, Li S. Eur J Surg Oncol. 2010 May;36(5):501-6. Doi: 10.1016/J.Ejso.2010.01.007. Epub 2010 Feb 23. The Low Expression Of Cd80 Correlated With The Vascular Endothelial Growth Factor In Esophageal Cancer Tissue.
Zhou L, Zhang K, Li J, Cui X, Wang A, Huang S, Zheng S, Lu Y, Chen W. Reprod Toxicol. 2013 Jun;37:62-9. Doi: 10.1016/J.Reprotox.2013.02.001. Epub 2013 Feb 11. Inhibition Of Vascular Endothelial Growth Factor-Mediated Angiogenesis Involved In Repro...
Chen J, Wei D, Zhuang H, Qiao Y, Tang B, Zhang X, Wei J, Fang S, Chen G, Du P, Huang X, Jiang W, Hu Q, Hua Zc. Mol Cell Proteomics. 2011 Jun;10(6):M111.009399. Doi: 10.1074/Mcp.M111.009399. Epub 2011 Apr 7. Proteomic Screening Of Anaerobically Reg...
Fu Z, Chen D, Cheng H, Wang F. Med Sci Monit. 2015 Jan 27;21:318-25. Doi: 10.12659/Msm.893265. Hypoxia-Inducible Factor-1?? Protects Cervical Carcinoma Cells From Apoptosis Induced By Radiation Via Modulation Of Vascular Endothelial Growth Factor ...
Liu Y, Lv L. J Huazhong Univ Sci Technolog Med Sci. 2004;24(5):470-2. Mechanism Of Elevated Vascular Endothelial Growth Factor Levels In Peritoneal Fluids From Patients With Endometriosis.
Li Xy, Lin Yc, Huang Wl, Hong Cq, Chen Jy, You Yj, Li Wb. Med Oncol. 2012 Jun;29(2):714-20. Doi: 10.1007/S12032-011-9904-1. Epub 2011 Mar 24. Zoledronic Acid Inhibits Proliferation And Impairs Migration And Invasion Through Downregulating Vegf And...
Ye X, Ren H, Zhang M, Sun Z, Jiang Ac, Xu G. Invest Ophthalmol Vis Sci. 2012 Jun 8;53(7):3481-9. Doi: 10.1167/Iovs.11-9076. Erk1/2 Signaling Pathway In The Release Of Vegf From M??ller Cells In Diabetes.
Shen J, Shen S, Das Un, Xu G. Lipids Health Dis. 2012 Jul 10;11:90. Doi: 10.1186/1476-511X-11-90. Effect Of Essential Fatty Acids On Glucose-Induced Cytotoxicity To Retinal Vascular Endothelial Cells.
Liu D, Cao G, Cen Y, Liu T, Peng W, Sun J, Li X, Zhou H. Int Immunopharmacol. 2013 Oct;17(2):237-44. Doi: 10.1016/J.Intimp.2013.06.002. Epub 2013 Jun 19. The Radiosensitizing Effect Of Cpg Odn107 On Human Glioma Cells Is Tightly Related To Its Ant...
Chai Zt, Kong J, Zhu Xd, Zhang Yy, Lu L, Zhou Jm, Wang Lr, Zhang Kz, Zhang Qb, Ao Jy, Wang M, Wu Wz, Wang L, Tang Zy, Sun Hc. Plos One. 2013 Oct 23;8(10):E77957. Doi: 10.1371/Journal.Pone.0077957. Ecollection 2013. Microrna-26A Inhibits Angiogenes...
Li Xq, Ouyang Zg, Zhang Sh, Liu H, Shang Y, Li Y, Zhen Ys. Cancer Biol Ther. 2014 Apr;15(4):398-408. Doi: 10.4161/Cbt.27626. Epub 2014 Jan 14. Synergistic Inhibition Of Angiogenesis And Glioma Cell-Induced Angiogenesis By The Combination Of Temozo...
Wang L, Zeng Y, Wang T, Liu H, Xiao H, Xiang H. Plos One. 2014 Jan 27;9(1):E86509. Doi: 10.1371/Journal.Pone.0086509. Ecollection 2014. Evaluating The Potential Bioactivity Of A Novel Compound Er1626.
Zhang H, Chen Y, Fan B, Wang W, Zhu W. Tumour Biol. 2015 May;36(5):3871-80. Doi: 10.1007/S13277-014-3029-Z. Epub 2015 Jan 11. Overexpression Of Vegf183 Promotes Murine Breast Cancer Cell Proliferation In Vitro And Induces Dilated Intratumoral Micr...
Das Kk, Jargar Jg, Saha S, Yendigeri Sm, Singh Sb. Indian J Pharmacol. 2015 May-Jun;47(3):285-91. Doi: 10.4103/0253-7613.157126. ??-Tocopherol Supplementation Prevents Lead Acetate And Hypoxia-Induced Hepatic Dysfunction.
Fan N, Zhang M, Xu K, Ke X, Ding Y, Wang D, Liu Y, Ning Y, Deng X, He H. Drug Alcohol Depend. 2015 Jul 1;152:57-61. Doi: 10.1016/J.Drugalcdep.2015.04.022. Epub 2015 May 8. Serum Level Of Vascular Endothelial Growth Factor Decreased In Chronic Keta...
Jiang Z, Han B, Li H, Yang Y, Liu W. Carbohydr Polym. 2015 Sep 20;129:1-8. Doi: 10.1016/J.Carbpol.2015.04.040. Epub 2015 Apr 27. Carboxymethyl Chitosan Represses Tumor Angiogenesis In Vitro And In Vivo.
Sancakdar E, Guven As, Uysal Eb, Deveci K, G??lt??rk E. Pediatr Infect Dis J. 2015 Aug;34(8):E200-5. Doi: 10.1097/Inf.0000000000000706. Important Of Angiopoietic System In Evaluation Of Endothelial Damage In Children With Crimean-Congo Hemorrhagic...
Lin L, Han Mm, Wang F, Xu Ll, Yu Hx, Yang Py. Cell Death Dis. 2014 Oct 23;5:E1488. Doi: 10.1038/Cddis.2014.392. Cxcr7 Stimulates Mapk Signaling To Regulate Hepatocellular Carcinoma Progression.
Guo Zy, Sun X, Xu Xl, Zhao Q, Peng J, Wang Y. Neural Regen Res. 2015 Apr;10(4):651-8. Doi: 10.4103/1673-5374.155442. Human Umbilical Cord Mesenchymal Stem Cells Promote Peripheral Nerve Repair Via Paracrine Mechanisms.
Jiang Z, Han B, Li H, Li X, Yang Y, Liu W. Carbohydr Polym. 2015 Jul 10;125:53-60. Doi: 10.1016/J.Carbpol.2015.02.039. Epub 2015 Feb 26. Preparation And Anti-Tumor Metastasis Of Carboxymethyl Chitosan.
Pan B, Ren H, Ma Y, Liu D, Yu B, Ji L, Pan L, Li J, Yang L, Lv X, Shen X, Chen B, Zhang Y, Willard B, He Y, Zheng L. Int J Cancer. 2012 Jul 1;131(1):70-82. Doi: 10.1002/Ijc.26341. Epub 2011 Aug 30. High-Density Lipoprotein Of Patients With Type 2 ...
Chen L, Xiao Z, Meng Y, Zhao Y, Han J, Su G, Chen B, Dai J. Biomaterials. 2012 Feb;33(5):1437-44. Doi: 10.1016/J.Biomaterials.2011.10.056. Epub 2011 Nov 10. The Enhancement Of Cancer Stem Cell Properties Of Mcf-7 Cells In 3D Collagen Scaffolds For...
Liu Lj, Zhang J, Xiao Zf, Dai B, Sun My, Chen L, Chen B. J Biomed Mater Res B Appl Biomater. 2014 May;102(4):772-80. Doi: 10.1002/Jbm.B.33058. Epub 2013 Oct 21. Three-Dimensional Collagen Scaffold Enhances The Human Adenoid Cystic Carcinoma Cancer...
Chen J, Xu J, Li J, Du L, Chen T, Liu P, Peng S, Wang M, Song H. Int Immunopharmacol. 2015 May;26(1):147-52. Doi: 10.1016/J.Intimp.2015.03.025. Epub 2015 Mar 31. Epigallocatechin-3-Gallate Attenuates Lipopolysaccharide-Induced Mastitis In Rats Via...
Yang Py, Rui Yc, Lu L, Li Tj, Liu Sq, Yan Hx, Wang Hy. Life Sci. 2005 Sep 30;77(20):2529-39. Time Courses Of Vascular Endothelial Growth Factor And Intercellular Adhesion Molecule-1 Expressions In Aortas Of Atherosclerotic Rats.
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Customer Q&As
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5 Customer Q&As for Mouse VEGF ELISA Kit PicoKine™
Question
Have other isoforms been tested for cross-reactivity with EK0541 kits?
Verified customer
Asked: 2019-09-25
Answer
The Mouse VEGF ELISA Kit PicoKine EK0541 is cross-reactive with their specific targets only.
Boster Scientific Support
Answered: 2019-09-26
Question
Q: how to thaw whole blood sample for VEGFA ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-04-07
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test VEGFA for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-04-07
Question
Q: can I use EDTA plasma as samples in Mouse VEGFA Picokine® ELISA Kit (Catalog # EK0541)?
Verified Customer
Verified customer
Asked: 2018-01-27
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of VEGFA causing degradation of its protein structure. VEGFA may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the VEGFA ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-01-27
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-07-06
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-07-06
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring VEGFA using the EK0541 Mouse VEGFA Picokine® ELISA Kit?
D. Banerjee
Verified customer
Asked: 2017-03-26
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2017-03-26
