Mouse VEGF ELISA Kit PicoKine®

Vegfa ELISA kit for Mouse

Mouse VEGF ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Vegfa in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0541
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

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Product Name

Mouse VEGF ELISA Kit PicoKine®

View all Vegfa ELISA kits

SKU/Catalog Number

EK0541

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse VEGF ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Vegfa in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse VEGF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0541)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A27-R190

Sensitivity

<2 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0541 is reactive to Vegfa in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0541 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Vegfa

Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1.The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12. The standard product used in this kit is recombinant mouse VEGF164, a 42KDa dimer.

Kit Components

Catalog Number Description Quantity
EK0541-CAP Anti-Mouse Vegfa Pre-coated 96-well strip microplate 1
EK0541-ST Mouse Vegfa Standard 2 vials, 10 ng/tube
EK0541-DA Mouse Vegfa Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0350.0740.1310.2110.3530.6601.2011.936

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse VEGF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)4017953840171574
Standard deviation1.929.4836.582.69.5748.79
CV (%)4.8%5.3%6.8%6.5%5.6%8.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Vegfa in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 140343537362.296.3%
Sample 21792041861971919.655%
Sample 353846349447149129.155.9%
*number of samples for each test n=16.

Gene/Protein Information For Vegfa (Source: Uniprot.Org, NCBI)

Uniprot ID

Q00731

Gene ID

22339

Gene Name

Vegfa

Full Name

Vascular endothelial growth factor A

Weight

25.283kDa

Superfamily

PDGF/VEGF growth factor family

Alternative Names

MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor Vegfa|V, VEGF12, VEGF16, VEGF18, Veg, Vegf, Vpf|vascular endothelial growth factor A|vascular endothelial growth factor A|vascular permeability factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Vegfa, check out the Vegfa Infographic

Vegfa infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Vegfa: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0541 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Histone demethylase KDM4C activates HIF1α/VEGFA signaling through the costimulatory factor STAT3 in NSCLC

Suppression of murine B-cell lymphoma growth by trichosanthin through anti-angiogenesis

IL-35 prevent bone loss through promotion of bone formation and angiogenesis in rheumatoid arthritis.

Modulation of Salmonella Tumor-Colonization and Intratumoral Anti-angiogenesis by Triptolide and Its Mechanism

Enhanced therapeutic effect by combination of tumor-targeting salmonella and endostatin in murine melanoma model

Inhibition of lung tumor growth in nude mice by siRNACD31 targeting PECAM‑1

A novel dressing seeded with embryonic artery CD133+ cells and loaded with the Sirt1 agonist SRT1720 accelerates the healing of diabetic ischemic ulcers

Antimetastatic and Antitumor Activities of Orally Administered NAX014 Compound in a Murine Model of HER2-Positive Breast Cancer

Serine racemase deficiency attenuates choroidal neovascularization and reduces nitric oxide and VEGF levels by retinal pigment epithelial cells

Autocrine VEGF signalling on M2 macrophages regulates PD‐L1 expression for immunomodulation of T cells

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6 Customer Q&As for Mouse VEGF ELISA Kit PicoKine®

Question

Have other isoforms been tested for cross-reactivity with EK0541 and EK0539?

Verified customer

Asked: 2019-09-25

Answer

The The Human VEGF ELISA Kit PicoKine EK0539 and Mouse VEGF ELISA Kit PicoKine EK0541 are cross-reactive with their specific targets only.

Boster Scientific Support

Answered: 2019-09-26

Question

Q: how to thaw whole blood sample for VEGFA ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-04-07

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test VEGFA for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-04-07

Question

Q: can I use EDTA plasma as samples in Mouse VEGFA Picokine® ELISA Kit (Catalog # EK0541)?

Verified Customer

Verified customer

Asked: 2018-01-27

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of VEGFA causing degradation of its protein structure. VEGFA may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the VEGFA ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-01-27

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-07-06

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2017-07-06

Question

Are the mouse, rat, and human VEGF kits truly specific for each species or do they cross react? Which isoforms of VEGF do the human, mouse and rat kit detect? Keyword: specificity

Verified Customer

Verified customer

Asked: 2017-06-08

Answer

Our VEGF Elisa kits are truly specific for each species, and they detect 165 amino acid form.

Boster Scientific Support

Answered: 2017-06-08

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring VEGFA using the EK0541 Mouse VEGFA Picokine® ELISA Kit?

D. Banerjee

Verified customer

Asked: 2017-03-26

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2017-03-26

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