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Mouse VEGF ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse VEGF. 96wells/kit, with removable strips. Cited in 48 publication(s).
Mouse VEGF ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0541 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Mouse |
| Sample Type: | cell culture supernates, serum and plasma(heparin, EDTA, citrate). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <2pg/ml |
| Assay Range: | 15.6pg/ml-1000pg/ml |
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Overview
| Product Name | Mouse VEGF ELISA Kit PicoKine™
See all Vegfa primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0541 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Mouse Vegf/VEGFA with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Vegf/VEGFA tissue specificity: In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression of VEGF continues in kidney glomeruli and choroid plexus in adults. |
| Cite This Product | Mouse VEGF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0541) |
Product Specs
| Reactivity/Species | Mouse |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, serum and plasma(heparin, EDTA, citrate). |
| Sensitivity | <2pg/ml |
| Assay Range | 15.6pg/ml-1000pg/ml |
| Immunogen | Expression system for standard: sf21, Immunogen sequence: A27-R190 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | Q00731 |
|---|---|
| Gene Name | Vegfa |
| Protein Name | Vascular endothelial growth factor A |
| Subcellular Localization | Isoform VEGF-1: Secreted. |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity). . |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Mouse VEGF ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0541-CAP | 96-well plate precoated with anti-Mouse Vegfa antibody | 1 |
| EK0541-ST | lyophilized recombinant Mouse Vegfa standard | 10ng/tube |
| EK0541-DA | biotinylated anti-Mouse Vegfa antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0541 Mouse VEGF ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.035 | 0.131 | 0.211 | 0.353 | 0.660 | 1.201 | 1.936 | 2.420 |
We measured random samples of Mouse VEGF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 40 | 179 | 538 | 40 | 171 | 574 |
| Standard deviation | 1.92 | 9.48 | 36.58 | 2.6 | 9.57 | 48.79 |
| CV(%) | 4.8% | 5.3% | 6.8% | 6.5% | 5.6% | 8.5% |
We measured the performance consistency of Mouse VEGF ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 40 | 34 | 35 | 37 | 36 | 2.29 | 6.3% |
| Sample 2 | 179 | 204 | 186 | 197 | 191 | 9.65 | 5% |
| Sample 3 | 538 | 463 | 494 | 471 | 491 | 29.15 | 5.9% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 48
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| Fan N, Zhang M, Xu K, Ke X, Ding Y, Wang D, Liu Y, Ning Y, Deng X, He H. Drug Alcohol Depend. 2015 Jul 1;152:57-61. Doi: 10.1016/J.Drugalcdep.2015.04.022. Epub 2015 May 8. Serum Level Of Vascular Endothelial Growth Factor Decreased In Chronic Keta... | PubMed ID 26003336 |
| Das Kk, Jargar Jg, Saha S, Yendigeri Sm, Singh Sb. Indian J Pharmacol. 2015 May-Jun;47(3):285-91. Doi: 10.4103/0253-7613.157126. ??-Tocopherol Supplementation Prevents Lead Acetate And Hypoxia-Induced Hepatic Dysfunction. | PubMed ID 26069366 |
| Zhang H, Chen Y, Fan B, Wang W, Zhu W. Tumour Biol. 2015 May;36(5):3871-80. Doi: 10.1007/S13277-014-3029-Z. Epub 2015 Jan 11. Overexpression Of Vegf183 Promotes Murine Breast Cancer Cell Proliferation In Vitro And Induces Dilated Intratumoral Micr... | PubMed ID 25577246 |
| Wang L, Zeng Y, Wang T, Liu H, Xiao H, Xiang H. Plos One. 2014 Jan 27;9(1):E86509. Doi: 10.1371/Journal.Pone.0086509. Ecollection 2014. Evaluating The Potential Bioactivity Of A Novel Compound Er1626. | PubMed ID 24475135 |
| Li Xq, Ouyang Zg, Zhang Sh, Liu H, Shang Y, Li Y, Zhen Ys. Cancer Biol Ther. 2014 Apr;15(4):398-408. Doi: 10.4161/Cbt.27626. Epub 2014 Jan 14. Synergistic Inhibition Of Angiogenesis And Glioma Cell-Induced Angiogenesis By The Combination Of Temozo... | PubMed ID 24424202 |
| Chai Zt, Kong J, Zhu Xd, Zhang Yy, Lu L, Zhou Jm, Wang Lr, Zhang Kz, Zhang Qb, Ao Jy, Wang M, Wu Wz, Wang L, Tang Zy, Sun Hc. Plos One. 2013 Oct 23;8(10):E77957. Doi: 10.1371/Journal.Pone.0077957. Ecollection 2013. Microrna-26A Inhibits Angiogenes... | PubMed ID 24194905 |
| Liu D, Cao G, Cen Y, Liu T, Peng W, Sun J, Li X, Zhou H. Int Immunopharmacol. 2013 Oct;17(2):237-44. Doi: 10.1016/J.Intimp.2013.06.002. Epub 2013 Jun 19. The Radiosensitizing Effect Of Cpg Odn107 On Human Glioma Cells Is Tightly Related To Its Ant... | PubMed ID 23791618 |
| Shen J, Shen S, Das Un, Xu G. Lipids Health Dis. 2012 Jul 10;11:90. Doi: 10.1186/1476-511X-11-90. Effect Of Essential Fatty Acids On Glucose-Induced Cytotoxicity To Retinal Vascular Endothelial Cells. | PubMed ID 22781401 |
| Ye X, Ren H, Zhang M, Sun Z, Jiang Ac, Xu G. Invest Ophthalmol Vis Sci. 2012 Jun 8;53(7):3481-9. Doi: 10.1167/Iovs.11-9076. Erk1/2 Signaling Pathway In The Release Of Vegf From M??ller Cells In Diabetes. | PubMed ID 22511624 |
| Li Xy, Lin Yc, Huang Wl, Hong Cq, Chen Jy, You Yj, Li Wb. Med Oncol. 2012 Jun;29(2):714-20. Doi: 10.1007/S12032-011-9904-1. Epub 2011 Mar 24. Zoledronic Acid Inhibits Proliferation And Impairs Migration And Invasion Through Downregulating Vegf And... | PubMed ID 21431960 |
| Liu Y, Lv L. J Huazhong Univ Sci Technolog Med Sci. 2004;24(5):470-2. Mechanism Of Elevated Vascular Endothelial Growth Factor Levels In Peritoneal Fluids From Patients With Endometriosis. | PubMed ID 15641695 |
| Fu Z, Chen D, Cheng H, Wang F. Med Sci Monit. 2015 Jan 27;21:318-25. Doi: 10.12659/Msm.893265. Hypoxia-Inducible Factor-1?? Protects Cervical Carcinoma Cells From Apoptosis Induced By Radiation Via Modulation Of Vascular Endothelial Growth Factor ... | PubMed ID 25623525 |
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| Ge Y, Ding Y, Zhang J, Li Z, Li Z. Int J Clin Exp Med. 2014 Oct 15;7(10):3578-82. Ecollection 2014. Effect Of Angiogenesis Inhibitor Su6668 In Combination With 5-Fu On Liver Metastasis From Transplantation Tumors Of Human Colorectal Cancer In Nude... | PubMed ID 25419401 |
| Wen D, Huang X, Zhang M, Zhang L, Chen J, Gu Y, Hao Cm. Plos One. 2013 Dec 3;8(12):E82336. Doi: 10.1371/Journal.Pone.0082336. Ecollection 2013. Resveratrol Attenuates Diabetic Nephropathy Via Modulating Angiogenesis. | PubMed ID 24312656 |
| Li Z, Ding Q, Ling LP, Wu Y, Meng DX, Li X, Zhang CQ. World J Gastroenterol. 2018 Feb 21;24(7):819-832. doi: 10.3748/wjg.v24.i7.819. Metformin attenuates motility, contraction, and fibrogenic response of hepatic stellate cells in vivo and in vitro... | PubMed ID 29467552 |
| Li M, Wang S, Wang S, Zhang L, Wu D, Yang R, Ji A, Li Y, Wang J. Exp Ther Med. 2017 Aug;14(2):1732-1738. doi: 10.3892/etm.2017.4651. Epub 2017 Jun 22. Occludin downregulation in high glucose is regulated by SSTR2 via the VEGF/NRP1/Akt signaling pa... | PubMed ID 28810643 |
| Sun Y,, Chen X, Zhang X, Shen X, Wang M, Wang X,, Liu WC, Liu CF, Liu J, Liu W, Jin X,. Front Mol Neurosci. 2017 Aug 14;10:257. doi: 10.3389/fnmol.2017.00257. eCollection 2017. β2-Adrenergic Receptor-Mediated HIF-1α Upregulation Mediates Blood Br... | PubMed ID 28855859 |
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| Microvesicles secreted from human multiple myeloma cells promote angiogenesis | PubMed ID 24374814 |
| Inhibition of lung tumor growth in nude mice by siRNACD31?targeting PECAM-1 | PubMed ID 24959215 |
| Relationship between single nucleotide polymorphisms in the 3?-untranslated region of the vascular endothelial growth factor gene and susceptibility to diabetic peripheral neuropathy in China | PubMed ID 25395956 |
| Efficiency of calcium phosphate composite nanoparticles in targeting Ehrlich carcinoma cells transplanted in mice | PubMed ID 26843980 |
| Induced migration of endothelial cells into 3D scaffolds by chemoattractants secreted by pro-inflammatory macrophages in situ | PubMed ID 28596912 |
| Modulation of Salmonella Tumor-Colonization and Intratumoral Anti-angiogenesis by Triptolide and Its Mechanism | PubMed ID 28740548 |
| A novel dressing seeded with embryonic artery CD133+ cells and loaded with the Sirt1 agonist SRT1720 accelerates the healing of diabetic ischemic ulcers | PubMed ID 29904408 |
| Neurotherapeutic Effect of Cord Blood Derived CD45 Hematopoietic Cells in Mice after Traumatic Brain Injury | PubMed ID 29274773 |
| Endostar down-regulates HIF-1 and VEGF expression and enhances the radioresponse to human lung adenocarcinoma cancer cells | PubMed ID 21567206 |
| Endostar, a modified recombinant human endostatin, exhibits synergistic effects with dexamethasone on angiogenesis and hepatoma growth | PubMed ID 21216091 |
| Autocrine VEGF signalling on M2 macrophages regulates PD-L1 expression for immunomodulation of T cells | PubMed ID 30456891 |
| Inhibition of tumor angiogenesis by interferon-γ by suppression of tumor-associated macrophage differentiation | PubMed ID 24854099 |
| Down-regulation of HPV18 E6, E7, or VEGF expression attenuates malignant biological behavior of human cervical cancer cells | PubMed ID 21222176 |
| Two tandem repeats of mHSP70407-426 enhance therapeutic antitumor effects of a recombined vascular endothelial growth factor (VEGF) protein vaccine | PubMed ID 29572180 |
| Intravenous injection of l-aspartic acid β-hydroxamate attenuates choroidal neovascularization via anti-VEGF and anti-inflammation | PubMed ID 30917905 |
| Serine racemase deficiency attenuates choroidal neovascularization and reduces nitric oxide and VEGF levels by retinal pigment epithelial cells | PubMed ID 28892569 |
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