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Product Info Summary
| SKU: | EK1195 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse WISP1/CCN4 ELISA Kit PicoKine®
View all WISP-1/CCN4 ELISA kits
SKU/Catalog Number
EK1195
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse WISP1/CCN4 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ccn4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse WISP1/CCN4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1195)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: T23-N367
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1195 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1195 is reactive to CCN4 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1195 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of WISP-1/CCN4
WISP1 (WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1195-CAP | Anti-Mouse CCN4 Pre-coated 96-well strip microplate | 1 |
| EK1195-ST | Mouse CCN4 Standard | 2 vials, 10 ng/tube |
| EK1195-DA | Mouse CCN4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.067 | 0.256 | 0.320 | 0.499 | 0.925 | 1.385 | 1.947 | 2.393 |
Data Example Images
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Mouse WISP1/CCN4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect WISP-1/CCN4, Dilution ratio of 1:1, concentration in serum and plasma is 1-2 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse WISP1/CCN4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 66 | 261 | 878 | 69 | 265 | 906 |
| Standard deviation | 4.29 | 16.7 | 60.58 | 5.58 | 18.81 | 67.95 |
| CV (%) | 6.5% | 6.4% | 6.9% | 8.1% | 7.1% | 7.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of WISP-1/CCN4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 66 | 72 | 72 | 73 | 70 | 2.77 | 3.9% |
| Sample 2 | 261 | 224 | 220 | 255 | 240 | 18.17 | 7.5% |
| Sample 3 | 878 | 877 | 881 | 905 | 885 | 11.49 | 1.2% |
Protein Target Info & Infographic
Gene/Protein Information For CCN4 (Source: Uniprot.Org, NCBI)
Gene Name
CCN4
Full Name
CCN family member 4
Weight
40.331kDa
Superfamily
CCN family
Alternative Names
CCN family member 4; CCN4; CCN4WISP1tc; WISP1; WISP-1; WISP1c; WISP1i; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1; Wnt-1-induced secreted protein; WNT1-inducible-signaling pathway protein 1 CCN4 WISP1, WISP1-OT1, WISP1-UT1, WISP1c, WISP1i, WISP1tc cellular communication network factor 4 CCN family member 4|WISP1 3UTR-associated RNA 1|WISP1 overlapping transcript 1 (non-protein coding)|WNT1 induced secreted protein 1|WNT1 inducible signaling pathway protein 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CCN4, check out the CCN4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCN4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse WISP1/CCN4 ELISA Kit PicoKine® (EK1195)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Mouse WISP1/CCN4 ELISA Kit PicoKine®
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Wisp1 using the EK1195 Mouse Wisp1 Picokine® ELISA Kit?
W. Robinson
Verified customer
Asked: 2020-07-06
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2020-07-06
Question
Q: how to thaw whole blood sample for Wisp1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-03-21
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Wisp1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-03-21
Question
Q: if the enzyme conjugated Wisp1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Wisp1 antigen?
D. Roberts
Verified customer
Asked: 2018-02-18
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-02-18
